H6254

Hyaluronidase from sheep testes

Type V, lyophilized powder, ≥1,500 units/mg solid

• Sinónimos: Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase
• Número de CAS: 37326-33-3
• Comisión internacional de enzimas: 3.2.1.35 (BRENDA, IUBMB)
• EC Number: 253-464-3
• MDL number: MFCD00131351
• UNSPSC Code: 12352204
• NACRES: NA.54

Propiedades

• biological source: sheep testis
• Quality Level: 200
• type: Type V
• form: lyophilized powder
• specific activity: ≥1,500 units/mg solid
• mol wt: 55 kDa
• application(s): diagnostic assay manufacturing
• storage temp.: −20°C

Descripción

General description

Hyaluronidase is present in high amount in testis as it aids fertilization of the mammalian egg. Mammalian hyaluronidase has a catalytic epidermal growth factor (EGF)-like domain and a C-terminal cysteine rich region.

Application

Hyaluronidase has been used:

• as a component of enzyme mix for the isolation of sertoli and germ cells from seminiferous tubules
• in the pre-treatment of deparaffinized adenomas sections for immunohistochemistry
• in the digestion of umbilical cord blood vessel for the generation of umbilical cord stromal stem cell lines

Biochem/physiol Actions

Hyaluronidase from sheep testis is commercially used to depolymerise hyaluronic acid and chondroitin-sulphate in connective tissue and cartilage respectively.

These enzymes randomly cleave β-N-acetylhexosamine-(1−4)-glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Unit Definition

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Información de seguridad

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)