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Key Documents

GERPN2209

ECL™ Western Blotting Detection Reagents

Cytiva RPN2209

Sinónimos:

ECL detection reagents, Western blot reagents

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About This Item

UNSPSC Code:
41105332
NACRES:
NA.32

feature

wetted part: no

packaging

pkg of 1 kit

manufacturer/tradename

Cytiva RPN2209

color

Detection reagent 1: Colorless Detection reagent 2: Colorless

storage temp.

2-8°C

General description

Amersham™ ECL™ Western Blotting Detection Reagents were the world′s first commercial chemiluminescent reagents for Western blotting and are still the most widely used. They are at least 10-fold more sensitive than colorimetric assays and produce excellent and reliable results every time.

These reagents use enhanced luminol-based detection which is suitable for all routine western blotting. ECL™ Western Blotting Detection Reagents in combination with Hybond® ECL™ membranes form a complete solution for Western blot applications.

Contents include the following:
GERPN2109, 62.5 mL each of ECL™ Detection Reagents 1 and 2 (1000 cm2)
GERPN2209, 125 mL each (2000 cm2)
GERPN2106, 250 mL each (4000 cm2)
GERPN2134, 375 mL each (6000 cm2)
Emission duration max. 2 h

Application

Western Blotting

Features and Benefits

  • High sensitivity, ~10 pg in model system.
  • Proven performance, the most frequently cited ECL™ Western Blotting Detection Reagents in publications
  • Detectable on Hyperfilm ECL™ or with CCD imager
  • Recommended mebrane Hybond ECL™

Components

Sufficient for detection of 2000 cm2 of membrane:
125 mL Detection reagent 1(color : colorless)
125 mL Detection reagent 2 (color: colorless)

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Amersham is a trademark of Cytiva
ECL is a trademark of Cytiva
Hybond is a registered trademark of Cytiva

Storage Class

12 - Non Combustible Liquids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Troubleshooting of Detection Methods of GST-tagged Proteins

Troubleshooting of Detection Methods of GST-tagged Proteins

Protocolos

Chemiluminescence Detection with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select

This page describes chemiluminescence detection of GST-tagged proteins with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select from GE Healthcare.

Detection of GST-tagged Proteins with Western Blot

This page describes how to monitor expression and purification of GST-tagged proteins by Western blot analysis using GE Healthcare products.

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