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Key Documents

GE29-0005-93

His Spintrap™ TALON®

Cytiva 29-0005-93

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

ligand

TALON® Superflow Cobalt-IMAC

manufacturer/tradename

Cytiva 29-0005-93

packaging

pkg of 50 x 100 μL

bed volume

100 μL

matrix

6% cross-linked agarose

particle size

60-160 μm

cleaning

2-14(Co2+-stripped medium)

working range

3-12(Co2+-stripped medium)

capacity

≤1 mg binding capacity(histidine-tagged)
 ≥20 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)

storage temp.

2-8°C

Categorías relacionadas

General description

His SpinTrap™ Talon® is a single-use column for rapid screening with small samples of histidine-tagged proteins.

Application

His SpinTrap™ Talon® is designed for high purity, small-scale purification and screening of histidine-tagged Proteins from clarified cell lysates. His SpinTrap™ Talon® columns are used together with a standard microcentrifuge and purification takes approximately 10 minutes. The columns make parallel preparations of few or many small-scale cell lysates quick and easy. Prepacked columns enable highly reproducible column to column purification and yield, with RSD < 6%. Leakage of cobalt ions from Talon® Superflow medium is negligible.

Features and Benefits

  • Suitable for purification of multiple samples in parallel, such as screening.
  • Convenient, ready-to-use single-use spin column, prepacked with 100 μL Talon® Superflow cobalt-IMAC (immobilized metal affinity chromatography) medium offering a different selectivity compared to nickel-charged media.
  • With a microcentrifuge, one run takes about 10 minutes.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

SpinTrap is a trademark of Cytiva
Talon is a registered trademark of Clontech

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.

Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose® Products

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from GE Healthcare.

Protocolos

Purification of Histidine-Tagged Proteins using TALON® Superflow

This page shows how to purify histidine-tagged proteins using TALON Superflow from GE Healthcare.

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