GE28-9365-50
GSTPrep™ Fast Flow 16/10
Cytiva 28-9365-50
About This Item
Productos recomendados
ligand
glutathione and 10-carbon linker arm
packaging
pkg of 20 mL
manufacturer/tradename
Cytiva 28-9365-50
parameter
<10 mL/min flow rate
1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi
bed size
16 mm × 100 mm
bed volume
20 mL
column I.D.
16 mm
matrix
highly cross-linked 4% agarose
average diameter
90 μm
cleaning
3-12
working range
3-12
capacity
>10 mg/mL binding capacity
General description
Application
Features and Benefits
- Fast and easy one-step, scale-up purification of GST-tagged Proteins.
- Binds approx. 200 mg recombinant GST/column
- Mild elution conditions preserving Protein antigenicity and function.
- Simple operation with a pump or chromatography system, such as ÄKTA® design.
Storage and Stability
Analysis Note
Other Notes
Legal Information
signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
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Artículos
This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from GE Healthcare.
This page shows troubleshooting methods for purification of GST-tagged proteins using GE Healthcare products.
This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from GE Healthcare.
This page describes characteristics of Glutathione Sepharose and HiTrap Benzamidine FF (high sub) media and columns from GE Healthcare.
Protocolos
This page how to remove GST tags by enzymatic cleavage using GE Healthcare products.
How to perform a separation with GST fusion proteins which are affinity chromatography tags used in GST MicroSpin Purifcation Module, GSTrap FF, GSTPrep FF 16/10, Glutathione Sepharose 4 Fast Flow and Glutathione Sepharose 4B products from GE Healthcare.
This page shows how to purify GST-tagged proteins using GSTrap HP, GSTrap FF and GSTrap 4B columns from GE Healthcare.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.
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