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Key Documents

GE17-5087-01

Sigma-Aldrich

HiPrep™ 26/10 Desalting

Cytiva 17-5087-01, column L × I.D. 10 cm × 26 mm, 17-132 μm particle size (dry)

Sinónimos:

Desalting Column, HiPrep Desalting Column

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

material

polypropylene (PP)

mol wt

1000-5000 Da (globular proteins)

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 17-5087-01

storage condition

dry media

parameter

<15 mL sample volume
<31 mL/min flow rate
0.15 Mpa (22 psi)

bed volume

53 mL

column L × I.D.

10 cm × 26 mm

matrix

cross-linked dextran
polymeric

matrix active group

dextran-epichlorohydrin phase

particle size

17-132 μm (dry)

cleaning

2-13

working range

2-13

separation technique

size exclusion (SEC)

Categorías relacionadas

General description

HiPrep™ 26/10 Desalting is a convenient, easy-to-use column prepacked 53 mL column with Sephadex® G-25 Fine for desalting and buffer exchange.

Features and Benefits

  • Provide reliable and reproducible desalting and buffer exchange with sample sizes of up to 15 mL per column.
  • Replaces dialysis for desalting and therefore saves time and reduces labor.
  • Compatible with a laboratory pump or chromatography system such as AKTA design.
  • Two or four columns can be used in series for sample sizes of 30 to 60 mL.

Suitability

All commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 24 % ethanol, 30 % porpanol, 30 % acetonitrile
for buffer exchange prior to or after different chromatographic steps.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

HiPrep is a trademark of Cytiva
Sephadex is a registered trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Automated Purification of Antibodies Using ÄKTA Chromatography Systems

This page describes automated purification of antibodies using ÄKTA chromatography systems.

Performing a purification and on-column refolding of an insoluble histidine-tagged protein

This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap FF columns and ÄKTAprime from GE Healthcare.

General Considerations for Purification of GST-tagged Protein

This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.

IgG Antibody Purification with HiTrap® Protein G HP Columns

This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from GE Healthcare.

Ver todo

Protocolos

Affinity Chromatography in a Purification Strategy (CIPP)

AC separates proteins on the basis of a reversible interaction between a protein (or group of proteins) and a specific ligand coupled to a chromatography matrix. With such high selectivity and hence high resolution for the protein(s) of interest, purification levels in the order of several thousand-fold with high recovery of active material are achievable.

Tag Removal by Enzymatic Cleavage

This page how to remove histidine tags by enzymatic cleavage using GE Healthcare products.

Conditioning Purified Membrane Proteins

This page shows how to condition membrane proteins for further analysis with products from GE Healthcare.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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