GE17-0780-01
Protein A-Sepharose™ CL-4B
Cytiva 17-0780-01, pack of 1.5 g
Sinónimos:
Protein A Sepharose, Sepharose CL-4B
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
UNSPSC Code:
41106500
NACRES:
NA.56
Productos recomendados
ligand
native protein A
packaging
pack of 1.5 g
manufacturer/tradename
Cytiva 17-0780-01
storage condition
(20% Ehtanol)
matrix
4% cross-linked agarose
particle size
45-165 μm
average diameter
90 μm
cleaning
2-10
working range
3-9
capacity
~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)
storage temp.
2-8°C
Categorías relacionadas
General description
Protein A Sepharose™ CL-4B is a classic, well documented medium for immunoglobin purification and fractionation, and is suitable for immunoprecipitation procedures.
Protein A binds to the Fc region of immunoglobulins through interactions with the heavy chain. The binding of protein A has been well documented for IgG from a variety of mammalian species and for some IgM and IgA as well. Protein A Sepharose™ CL-4B has been used as a powerful tool to isolate and purify classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Since only the Fc region is involved in binding, the Fab region is available for binding antigen.
Protein A binds to the Fc region of immunoglobulins through interactions with the heavy chain. The binding of protein A has been well documented for IgG from a variety of mammalian species and for some IgM and IgA as well. Protein A Sepharose™ CL-4B has been used as a powerful tool to isolate and purify classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Since only the Fc region is involved in binding, the Fab region is available for binding antigen.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
Features and Benefits
- Well documented for immunoglobulin purification and fractionation
- Strong binding to Fc region of IgG
- High capacity - 20 mg human IgG/mL.
- Suited for immunoprecipitation procedures
Storage and Stability
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Sepharose is a trademark of Cytiva
Storage Class
13 - Non Combustible Solids
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
Daniel L Galvan et al.
The Journal of clinical investigation, 129(7), 2807-2823 (2019-05-08)
Phosphorylation of Dynamin-related protein1 (Drp1) represents an important regulatory mechanism for mitochondrial fission. Here we established the role of Drp1 Serine 600 (S600) phosphorylation on mitochondrial fission in vivo, and assessed the functional consequences of targeted elimination of the Drp1S600
Anahita Nejatfard et al.
Cell reports, 37(3), 109840-109840 (2021-10-24)
Nearly one-third of proteins are initially targeted to the endoplasmic reticulum (ER) membrane, where they are correctly folded and then delivered to their final cellular destinations. To prevent the accumulation of misfolded membrane proteins, ER-associated degradation (ERAD) moves these clients
Vishal Kothari et al.
PloS one, 17(5), e0266472-e0266472 (2022-05-07)
Fetuin-A (Fet-A) is a liver-secreted phosphorylated protein, known to impair insulin signaling, which has been shown to be associated with obesity, insulin resistance, and incident diabetes. Fet-A interacts with the insulin-stimulated insulin receptor (IR) and inhibits IR tyrosine kinase activity
Easwaran Ramamurthy et al.
Frontiers in molecular neuroscience, 15, 948456-948456 (2023-01-24)
We profile genome-wide histone 3 lysine 27 acetylation (H3K27ac) of 3 major brain cell types from hippocampus and dorsolateral prefrontal cortex (dlPFC) of subjects with and without Alzheimer's Disease (AD). We confirm that single nucleotide polymorphisms (SNPs) associated with late
Ana Isabel de Los Santos-Velázquez et al.
Current biology : CB, 27(21), 3248-3263 (2017-10-24)
The nucleolus plays a pivotal role in multiple key cellular processes. An illustrative example is the regulation of mitotic exit in Saccharomyces cerevisiae through the nucleolar sequestration of the Cdc14 phosphatase. The peculiar structure of the nucleolus, however, has also
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico