Saltar al contenido
Merck

G8021

Sigma-Aldrich

Monoclonal Anti-β-Galactosidase antibody produced in mouse

clone GAL-13, ascites fluid

Sinónimos:

Monoclonal Anti-β-Galactosidase

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

GAL-13, monoclonal

contains

15 mM sodium azide

species reactivity

bacteria

technique(s)

dot blot: suitable using soluble β-galactosidase
immunocytochemistry: suitable
indirect ELISA: 1:2,000 using mouse primary antibody, bridging antibody and Escherichia coli β-galactosidase

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

Escherichia coli ... lacZ(1036228) , lacZ(1036228)
Escherichia coli K12 ... Lacz(945006)

¿Está buscando productos similares? Visita Guía de comparación de productos

General description

Monoclonal Anti-β-Galactosidase (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. β −galactosidase is a E coli protein and is a tetramer with 1023 amino acids.

Specificity

The antibody may be used for amplification in immunoenzymatic staining by preparing a β-galactosidase anti-β-galactosidase (BGABG) soluble complex. It is not recommended for immunoblotting; it does not recognize denatured or reduced β-galactosidase.

Immunogen

β-galactosidase from E. coli

Application

Monoclonal Anti-β-Galactosidase antibody produced in mouse has been used in:

  • immunostaining and histochemistry
  • immunostaining and microscopy
  • immunohistochemistry
  • immunofluorescence staining
  • enzyme linked immunosorbent assay(ELISA)
  • immuno dot blot

Biochem/physiol Actions

β −galactosidase substrate is lactose. Synthetic substrates like o-nitrophenyl-β-d-galactopyranoside (oNPG) or p-nitrophenyl-β-d-galactopyranoside (pNPG) are used in enzymatic assays. It is also used as marker in studying gene expression. The use of monoclonal β -galactosidase anti- β -galactosidase (BGABG) soluble complex in the unlabeled antibody-enzyme method results in an intense signal with a very low background.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

¿No encuentra el producto adecuado?  

Pruebe nuestro Herramienta de selección de productos.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Effect of alphavbeta3 Integrin Expression and Activity on Intraocular Pressure
Faralli JA, et al.
Investigative Ophthalmology & Visual Science, 60(5), 1776-1788 (2019)
L Heslop et al.
Gene therapy, 8(10), 778-783 (2001-06-23)
Myoblast transplantation is a potential therapeutic approach for the genetic modification of host skeletal muscle tissue. To be considered an effective, long-lived method of delivery, however, it is essential that at least a proportion of the transplanted cells also retain
Katrina M Williams et al.
Scientific reports, 7, 39817-39817 (2017-01-04)
Methamphetamine (Meth) is a psychomotor stimulant strongly associated with increases in sexual drive and impulse in both men and women. These changes in sexual motivation have a greater impact on women due to their likelihood of facing the greater burden
Transcription factor TLX1 controls retinoic acid signaling to ensure spleen development
Lenti E, et al.
The Journal of Clinical Investigation, 126(7), 2452-2464 (2016)
Expanding the utility of beta-galactosidase complementation: piece by piece
Broome AM, et al.
Molecular Pharmaceutics, 7(1), 60-74 (2009)

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico