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Merck

G1875

Sigma-Aldrich

β-Galactosidase from bovine liver

Grade III, lyophilized powder, ≥0.15 units/mg protein

Sinónimos:

β-D-Galactoside galactohydrolase

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About This Item

Número de CAS:
Comisión internacional de enzimas:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

bovine liver

Quality Level

type

Grade III

form

lyophilized powder

specific activity

≥0.15 units/mg protein

composition

Protein, ≥40%

shipped in

wet ice

storage temp.

−20°C

Categorías relacionadas

Application

β-galactosidase was used in the production of a stabilized, single reagent for alcohol analysis.

Biochem/physiol Actions

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

Unit Definition

One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.

substrate

Referencia del producto
Descripción
Precios

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificados de análisis (COA)

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Stabilization of Analytical Enzymes Using a Novel Polymer-Carbohydrate System and the Production of a Stabilized, Single Reagent for Alcohol Analysis
Timothy D. Gibson, John Higgins, John R. Woodward
Analyst, 117, 1293-1297 (1992)
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D H Juers et al.
Protein science : a publication of the Protein Society, 8(1), 122-136 (1999-04-21)
Beta-galactosidase (lacZ) from Escherichia coli is a 464 kDa homotetramer. Each subunit consists of five domains, the third being an alpha/beta barrel that contains most of the active site residues. A comparison is made between each of the domains and
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