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Merck

F3022

Sigma-Aldrich

Anti-β-actina monoclonal

clone AC-15, purified from hybridoma cell culture

Sinónimos:

Monoclonal Anti-β-Actin

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

AC-15, monoclonal

form

buffered aqueous solution

mol wt

antigen 42 kDa

species reactivity

sheep, carp, feline, chicken, rat, mouse, Hirudo medicinalis, rabbit, canine, pig, human, bovine, guinea pig

should not react with

Dictyostelium discoideum

technique(s)

direct immunofluorescence: 1:250 using cultured human and chicken fibroblasts

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ACTB(60)
mouse ... Actb(11461)
rat ... Actb(81822)

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General description

En la tinción de criosecciones tisulares ultrafinas de molleja de pollo, el anticuerpo marca los cuerpos densos y los canales longitudinales que unen cuerpos densos consecutivos que también están ocupados por desmina y la placa densa asociada a la membrana. No tiñe los músculos cardiaco y esquelético adultos excepto las trazas debidas a la contaminación de la muestra con células no musculares o si se está utilizando tejido embrionario.
Monoclonal Anti-b-Actin (mouse IgG1 isotype) is derived from the AC-15 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. ACTB (actin beta) gene codes for β-actin. It is a cytoskeletal housekeeping protein. It is present in the cytoplasm. This gene is located on human chromosome 7p22.1. Actin is a eukaryotic protein. It belongs to the major cytoskeletal protein family.

Immunogen

péptido N-terminal de la actina β-citoplasmática ligeramente modificado, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugado con KLH.

Application

El anticuerpo monoclonal anti-actina de ratón se utilizó como control de carga para el análisis mediante inmunoelectrotransferencia de las proteínas inmunoprecipitadas de cocultivos de los ganglios radiculares dorsales de rata.
La anti-actina monoclonal de ratón se utilizó como un control de carga para el análisis mediante transferencia Western de lisados proteicos de hígado de rata.
Anti-β-Actin-FITC antibody, Mouse monoclonal has been used in:
  • western blotting
  • immunohistochemistry
  • flow cytometry
  • direct immunofluorescent staining
  • dual immuno-fluorescent staining

Biochem/physiol Actions

β-Actin modulates cell growth, migration and the G-actin pool. Mutations in ACTB leads to pleiotropic developmental disorder.
Actins are majorly associated with cell motility. It is ubiquitously present in cytoplasm and involved in a myriad of cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. It is expressed in mammals and birds and at least six isoforms have been identified.
Actins is a eukaryotic protein. It belongs to the major cytoskeletal protein family and majorly associated with cell motility. It is ubiquitously present in cytoplasm and involved in a myriad of cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. It is expressed in mammals and birds and at least six isoforms have been identified.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificados de análisis (COA)

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