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Merck
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E6754

Sigma-Aldrich

Epoxy-activated−Sepharose™ 6B

lyophilized powder

Sinónimos:

Epoxy-activated-Agarose

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About This Item

MDL number:
MFCD00163561
UNSPSC Code:
41106500
NACRES:
NA.56

form

lyophilized powder

extent of labeling

19-40 μmol per mL

technique(s)

affinity chromatography: suitable

matrix

Sepharose 6B

matrix active group

epoxy

matrix attachment

through epoxy to hydroxyl

matrix spacer

12 atoms

swelling

1 g swells to 3.0-5.0 mL

particle size

45—165 μm

storage temp.

2-8°C

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General description

when ligands are coupled to free epoxy groups. Linkage is a stable, uncharged ether.

Application

Epoxy-activated-Sepharose™ is used in affinity chromatography, protein chromatography, and activated/functionalized matrices. Epoxy-activated-Sepharose™ has been used to obtain a monoclonal antibody against the prokaryotically expressed foot-and-mouth disease virus (FMDV) non-structural protein (NSP) 3B as well as to study B meningococcal disease.

Other Notes

Ligand: 1,4-bis(2:3-Epoxypropoxy)butane
Attachment: one epoxy group
Spacer: provides a 12-atom spacer when ligands are coupled to free epoxy groups. Linkage is a stable, uncharged ether.

Physical form

Lyophilized powder

Legal Information

Sepharose is a trademark of Cytiva

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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C A O'Brian et al.
Cancer research, 48(13), 3626-3629 (1988-07-01)
We have previously demonstrated that tamoxifen and related triphenylethylene compounds are potent inhibitors of protein kinase C (PKC). The present study demonstrates that PKC binds specifically and reversibly to the antiestrogen N-didesmethyltamoxifen when the drug is coupled to CNBr-activated agarose
Dong Li et al.
Veterinary research communications, 34(5), 445-457 (2010-06-01)
A monoclonal antibody, 3BIgG, against the prokaryotically expressed foot-and-mouth disease virus (FMDV) non-structural protein (NSP) 3B was obtained. The 3BIgG-sepharose conjugant (3BmAb-6BFF) was prepared by adding the purified 3BIgG into epoxy-activated sepharose 6BFF, incubating with the inactivated FMDV, and then
Adam Frankel et al.
RNA (New York, N.Y.), 9(7), 780-786 (2003-06-18)
The universal genetic code links the 20 naturally occurring amino acids to the 61 sense codons. Previously, the UAG amber stop codon (a nonsense codon) has been used as a blank in the code to insert natural and unnatural amino
Kasim Abass Askar et al.
Applied biochemistry and biotechnology, 165(1), 336-346 (2011-04-19)
The purpose of this study was to develop a protocol for the purification of acetylcholinesterase (AChE, acetylcholine acetylhydrolase, E.C.3.1.1.7) enzyme and to extend a purification method for further enzyme characterization. A further aim was to study whether the edrophonium's pharmacologic
A N Jina et al.
Biochemistry, 29(21), 5203-5209 (1990-05-29)
To synthesize an affinity matrix for isolation of D-myo-inositol 1,4,5-trisphosphate binding proteins, racemic 3-cyclohexene-1-carboxaldehyde was oxidized and converted to a mixture of trans-3,4-di-hydroxycyclohexane-1-carboxylic acid methyl ester isomers, which was phosphorylated and separated into (+-)-(1R,3R,4R)- and (+-)-(1R,3S,4S)-trans-3,4-bis[(diphenoxyphosphoryl)oxy]cyclohex an e-1- carboxylic acid
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