CS2000
SARS-CoV-2 (COVID-19) Inhibitors Screening Kit
Based on SPIKE - ACE2 Binding Assay, Colorimetric, sufficient for 200 tests
Sinónimos:
ACE2, COVID-19, S protein, SARS-CoV-2, Spike protein
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
Productos recomendados
General description
designed to simplify the identification and characterization of SARS-CoV-2 inhibitors.
Application
The SARS-CoV-2 Inhibitors Screening Kit is a convenient tool, designed to simplify the identification and characterization of SARS-CoV-2 inhibitors.The crutial interaction between the SARS-CoV-2 and human cells accrues through the protein-protein interaction of human receptor ACE2 and viral protein Spike – S protein. The receptor binding domain (RBD) of S protein is the domain that specifically binds to ACE2. Our SARS-CoV-2 Inhibitors Screening Kit includes both human ACE protein and viral RBD of S protein. The kit is based on a colorimetric ELISA, where biotinylated ACE2 (ACE2-biotin) and Extravidin®-peroxidase are used to detect the ACE2:RBD interaction. One molecule of Extravidin® binds four molecules of biotin by a non-covalent interaction that is essentially irreversible. Binding visualization is achieved by using o-Phenylenediamine dihydrochloride (OPD), a peroxidase substrate with absorbance at 450 nm.
Inhibitors and neutralizing antibodies against RBD that interfere with the ACE2:RBD interaction will result in reduced colorimetric signal.
Inhibitors and neutralizing antibodies against RBD that interfere with the ACE2:RBD interaction will result in reduced colorimetric signal.
Biochem/physiol Actions
SARS-CoV-2 - Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) belongs to Coronaviridae (CoVs) - a large family of viruses that usually cause mild to moderate upper-respiratory tract diseases.1 CoVs are single-stranded RNA viruses. They have a high mutation rate and are therefore highly diverse.1 Their hosts include human and non-human mammals, as well as birds.1
SARS-CoV-2 infects cells which express angiotensin converting enzyme 2 (ACE2).2 ACE2 binds to the viral envelope-associated spike (S) protein as a cellular receptor, following proteolytic cleavage of both S and ACE2 by serine proteases.3 S protein is believed to be the target of neutralizing antibodies because it is the main trans-membrane glycoprotein responsible for receptor-binding and virion entry.4
The S protein is a trimeric protein. During the binding to ACE2, S protein undergoes a substantial structural rearrangement to facilitate the fusion of the viral membrane with the host cell membrane.5 The receptor binding domain (RBD) of S protein is the domain that specifically binds to ACE2. RBD is the major target for neutralizing antibodies for coronaviruses6, and anti-RBD antibodies likely correlate with virus neutralization.7
SARS-CoV-2 infects cells which express angiotensin converting enzyme 2 (ACE2).2 ACE2 binds to the viral envelope-associated spike (S) protein as a cellular receptor, following proteolytic cleavage of both S and ACE2 by serine proteases.3 S protein is believed to be the target of neutralizing antibodies because it is the main trans-membrane glycoprotein responsible for receptor-binding and virion entry.4
The S protein is a trimeric protein. During the binding to ACE2, S protein undergoes a substantial structural rearrangement to facilitate the fusion of the viral membrane with the host cell membrane.5 The receptor binding domain (RBD) of S protein is the domain that specifically binds to ACE2. RBD is the major target for neutralizing antibodies for coronaviruses6, and anti-RBD antibodies likely correlate with virus neutralization.7
Features and Benefits
Our SARS-CoV-2 Inhibitors Screening Kit will support your research, enabling to screen and discover inhibitors as potential drugs for COVID-19
Supportive calculator (Click here to download a calculator excel file)
Legal Information
ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Skin Sens. 1 - STOT SE 3
target_organs
Respiratory system
Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico