CLLS1214
MCF10A CELLS HER2 (-/-) EXON 2
human female maMilliporeary glands (Source Disease: Fibrocystic Disease)
About This Item
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product name
MCF10A CELLS HER2 (-/-) EXON 2,
biological source
human female mammary glands (Source Disease: Fibrocystic Disease)
Quality Level
OMIM accession no.
storage temp.
−196°C
Gene Information
human ... ERBB2(2064) , HER2(2064)
General description
This cell line was derived from ATCC Catalog No. CRL-10317.
Application
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1:3-1:8
The base medium for this cell line is Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F-12, Cat. No. 51448C. To make the complete growth medium, add the following components to the base medium: horse serum, Cat. No. H1270, to a final concentration (v/v) of 5%; cholera toxin, Cat. No. C8052, to a final concentration of 1 ng/mL; human insulin, Cat. No. I9278, to a final concentration of 10 ug/mL; epidermal growth factor, Cat. No. E9644, to a final concentration of 10 ng/mL; and hydrocortisone, Cat. No. H6909, to a final concentration of 0.5 ug/mL.
Cell freezing medium - DMSO 1X, Cat. No. C6164.
Features and Benefits
Zinc finger nuclease (ZFN) modification on chromosome 17q21.1
Quality
Disclaimer
Solo componentes del kit
- MCF10A CELLS HER2 (-/-) EXON 2 1 vial
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Contenido relacionado
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