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CLLS1007

Sigma-Aldrich

SW48 Cells TP53 -/-

Human female colon, colorectal adenocarcinoma

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About This Item

UNSPSC Code:
41106514
NACRES:
NA.81

Nombre del producto

SW48 Cells TP53 -/-,

biological source

human female colon (Source Disease: Colorectal adenocarcinoma)

Quality Level

200

OMIM accession no.

191170

storage temp.

−196°C

Gene Information

human ... TP53(7157)

General description

SW48 Cells TP53 -/- are colorectal adenocarcinoma, epithelial cells, from a human caucasian female (aged 82 years), with a ZFN knock out modification.

Application

Media Renewal changes one to two times per week
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1:2 to 1:6
The base medium for this cell line is DMEM, Cat. No. D5671. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10%, Sodium pyruvate, Cat. No. S8636, at a final concentration of 1 mM, and L-glutamine, Cat. No. G7513, at a final concentration of 2mM.
The cell freezing medium is DMSO 1X (Cat. No. C6164)

Features and Benefits

These SW48 cells are adherent, with a doubling time of approx. 35 hours.
Zinc finger nuclease (ZFN) knock out on chromosome 17p13.1

Quality

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS
  • SW48 Cells TP53 -/- 1 vial
  • SW48 Control Cells 1 vial

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