ATDC5

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 99072806, mouse unknown/unspecified, Epithelial-like

• Sinónimos: ATDC-5 Cells
• UNSPSC Code: 41106514

Propiedades

• biological source: mouse unknown/unspecified
• packaging: tube of 5 μg 99072806-DNA-5UG; pkg of vial of cells 99072806-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial-like
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Mouse 129 teratocarcinoma AT805 derived

Cell Line Description

The chondrogenic cell line ATDC5 has been derived from teratocarcinoma AT805. Cells showed a sequential transition of phenotype in vitro. They encompass stages from mesenchymal condensation to calcification. Bone morphogenetic protein-2 (BMP-2) stimulated the sequential progression of early- and late-phase differentiation. Activation of parathyroid hormone (PTH)/PTH-related peptide (PTHrP) receptor resulted in inhibition of this differentiation capacity.

Application

ATDC5 has been used to study cartilage differentiation.

Culture Medium

DMEM: Ham′s F12 (1:1) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) i.e. seeding at 1-3 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA;CO₂; 37°C subculture every 2-3 days.

Other Notes

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