HEL92.1.7

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Propiedades

• biological source: human blood
• packaging: tube of 5 μg 92111706-DNA-5UG; pkg of vial of cells 92111706-1VL
• growth mode: Suspension
• karyotype: Not specified
• morphology: Lymphoblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Human Caucasian erythroleukaemia

Cell Line Description

Derived from 30 year old male Caucasian. Expresses ß-2 microglobulin and Ia antigen. Used in the study of erythroid cell differentiation and globin gene expression. Analogous to Friend cell leukaemia in mice. K-562 has similar characteristics.

Application

Erythroid cell differentiation studies, induced globin synthesis

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10
D13S317: 9,11
D16S539: 11
D5S818: 11
D7S820: 7
THO1: 7
TPOX: 11
vWA: 14,17

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Cell growth can be very slow on resuscitation. Stand the culture flask up overnight using 20% FBS.

Other Notes

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