OX-35 Cell Line from murine myeloma

clone 5, 89041184, Reactivity: Anti- CD4, rat

• UNSPSC Code: 41106514

Propiedades

• biological source: Mouse x Mouse Hybridoma
• clone: 5, monoclonal
• ImmunoDon: BALB/c mouse spleen
• Myeloma: NS0/1
• Reactivity: Anti-CD4, rat
• technique(s): cell culture | mammalian: suitable
• isotype: IgG2a
• shipped in: dry ice

Descripción

Cell Line Origin

Mouse NS0/U x Mouse BALB/c spleen

Cell Line Description

The antibody is directed against the CD4 antigen on rat T cells and macrophages. The specificity is the same as that of W3/25, though their binding is non-competitive. OX-35 will compete with OX-36.

Immunogen

T blasts prepared in mixed lymphocyte reactions with purified rat T helper cells against irradiated spleen

Culture Medium

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Seed at 5x10,000 viable cells/ml. Subculture when cells reach 5x100,000-1x1,000,000 cells/ml (usually after 3-4 days); 5% CO₂; 37°C.

Other Notes

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Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.