RPMI 6666

87081807, human blood, Lymphoblast

• UNSPSC Code: 41106514

Propiedades

• product name: RPMI 6666, 87081807
• biological source: human blood
• growth mode: Suspension
• karyotype: 2n = 46
• morphology: Lymphoblast
• products: Immunoglobulin
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Human Caucasian lymphoblast, Hodgkins disease

Cell Line Description

Derived from a 29 year old male Caucasian with Hodgkin′s disease. One of a large series of permanent lines from leukocytes in the peripheral blood of donors with and without malignancies. Has cell-associated EBV and produces immunoglobulins. This cell line was initially thought to have been derived from Hodgkin lymphoma, but is now known to be an EBV-positive B-lymphoblastoid cell line.

Application

Tumourigenicity studies

Culture Medium

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Other Notes

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