KNRK

87061001, rat kidney, Epithelial-like

• UNSPSC Code: 41106514

Propiedades

• product name: KNRK, 87061001
• biological source: rat kidney
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial-like
• products: Growth factors
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Normal rat kidney, Kirsten MSV transformed

Cell Line Description

Derived from normal rat kidney transformed by Kirsten sarcoma virus. Cells do not produce virus but carry the virus genome. In serum free medium the cell line produces growth factors capable of stimulating quiescent cells to grow in monolayers or in soft agar.

Application

Transformation, growth factors and virus studies: Kirsten MSV

Culture Medium

DMEM + 2mM Glutamine + 5-10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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