AC2

86061902, mouse blood, Lymphoblast

• UNSPSC Code: 41106514

Propiedades

• product name: AC2, 86061902
• biological source: mouse blood
• growth mode: Suspension
• karyotype: Not specified
• morphology: Lymphoblast
• products: Not specified
• receptors: Interleukin 3 (IL-3)
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Mouse lymphoblast

Cell Line Description

Derived from a mouse basophil clone and is dependent on Interleukin-3 (IL-3) for growth. IL-3 may be added in the form of Wehi conditioned media (10-20%). Culture densities between 10,000 and 100,000 cells/ml is necessary to retain cells in exponential growth phase.

Application

Growth factor studies, IL-2 assays

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 10,000 to 100,000 cells/ml; 5% CO₂; 37°C. Cells will rapidly die at high densities above 5x100,000 cells/ml. Culture very sensitive to agitation.

Other Notes

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