HT55 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA. 85061105, human colon, Epithelial

• Sinónimos: HT55 Cells
• UNSPSC Code: 41106514

Propiedades

• biological source: human colon
• description: Human colon carcinoma
• packaging: tube of 5 μg 85061105-DNA-5UG; vial of cells 85061105-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Human colon carcinoma

Cell Line Description

Human colon epithelial cell line

Application

Tumourigenicity studies

Packaging

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 9,13
D16S539: 14
D5S818: 11,12
D7S820: 11,13
THO1: 8,9.3
TPOX: 8,9
vWA: 14,17

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA and incubate until cell sheet peels off. Cells grow in islands, not monolayers; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.