3T3 Swiss Albino

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85022108, mouse embryo, Fibroblast

• UNSPSC Code: 41106514

Propiedades

• biological source: mouse embryo
• packaging: tube of 5 μg 85022108-DNA-5UG; pkg of vial of cells 85022108-1VL
• growth mode: Adherent
• karyotype: 2n = 40; hypertriploid
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Descripción

General description

Product has lost contact inhibition

Cell Line Origin

Mouse Swiss Albino embryo fibroblast

Cell Line Description

Evolved under conditions favouring retention of the property of contact inhibition of cell proliferation; they exhibit much more contact inhibition than diploid fibroblasts freshly plated in culture. Readily transformed by polyoma and SV40 viruses resulting in reduced sensitivity to contact inhibition. Cells have now lost their contact inhibition.

Application

Contact inhibition and virus studies: polyoma and SV40 viruses

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 5x1,000 to 1x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.