RAJI Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85011429, human lymph, Lymphoblastoid

• Sinónimos: GM04671 Cells, P1-Raji Cells
• UNSPSC Code: 41106514

Propiedades

• biological source: human lymph
• description: Human Black, Burkitt′s lymphoma
• packaging: tube of 5 μg 85011429-DNA-5UG; pkg of vial of cells 85011429-1VL
• growth mode: Suspension
• karyotype: 2n = 46, diploid
• morphology: Lymphoblastoid
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Human Burkitt′s lymphoma

Cell Line Description

Established in 1963 from Burkitt′s lymphoma in an 11 year old male. Growth is in the form of single cells without attachment and as macroscopically visible clumps containing many hundreds of cells. Resistant to VSV. This cell line carries the latent Epstein-Barr Virus (EBV) genome and is positive for EBNA. RAJI is sometimes referred to as a ′non-producer′; the EBV genome carries deletions attributed to preventing the formation of virus particles. Ethnicity: Black.

Application

Resistance and virus studies: vesicular stomatitis virus (VSV).
RAJI Cell Line human has been used to investigate the transport of V. parahaemolyticus across the Payer′s patch of the intestinal epithelium and it has been co-cultured with Caco-2 (human colorectal adenocarcinoma cell-line) monolayers to study bacterial translocation.

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 13
D16S539: 8,11
D5S818: 10,13
D7S820: 10
THO1: 6,7
TPOX: 8,13
vWA: 16,19

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Other Notes

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