BHK21C13-2P

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 84111301, hamster kidney, Not specified

• UNSPSC Code: 41106514

Propiedades

• biological source: hamster kidney
• packaging: tube of 5 μg 84111301-DNA-5UG; pkg of vial of cells 84111301-1VL
• growth mode: Suspension
• karyotype: 2n = 44, pseudodiploid
• morphology: Not specified
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Hamster Syrian kidney

Cell Line Description

Derived from the parent line BHK-21 (Clone-13) (Sigma Catalogue number. 85011433). The cells have been adapted to growth in suspension and have the same virus susceptibility as the parent line.

Application

Virus replication studies

Culture Medium

GMEM + 2mM Glutamine + 5% Tryptose Phosphate Broth + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 2-9x100,000 cells/ml (split ratio of 1:3 to 1:6); 5% CO₂; 37°C. Cells need to be grown in a techni-stirrer or they may start to re-attach.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.