MM466

12070507

• Sinónimos: MM 466
• UNSPSC Code: 41106514

Propiedades

• biological source: human skin (metastasis)
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• receptors: Not specified
• relevant disease(s): metastasis

Descripción

Cell Line Origin

Human melanoma metastatic site lymph node

Cell Line Description

mM466 is a human melanoma derived from a metastatic site (lymph node). Cell Bank Australia reference number CBA-1353.

DNA Profile

Not specified

Culture Medium

RPMI 1640 (R0883) (with 2 mM L-Glutamine (G7513) + 25mM HEPES) + 10% FBS.

Subculture Routine

Optimal split ratio 1:2-1:4 (seeding density 1.4 x 10⁴ cells/cm²). Cells incubated at 37 °C with 5% CO₂. Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 5 min. Medium change twice weekly. The cell line MM466 may take up to 72 hours to recover from thaw.

Other Notes

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Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Peter Parsons - Queensland Institute of Medical Research, Australia