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C7902

Sigma-Aldrich

Cloruro de calcio dihydrate

BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%

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About This Item

Fórmula empírica (notación de Hill):
CaCl2 · 2H2O
Número de CAS:
10035-04-8
Peso molecular:
147.01
EC Number:
233-140-8
MDL number:
MFCD00149613
UNSPSC Code:
12352302
PubChem Substance ID:
24892994
NACRES:
NA.71
assay:
≥99.0%
grade:
reagent
form:
powder
solubility:
H2O: 294 mg/mL, clear, colorless

grade

reagent

product line

BioReagent

assay

≥99.0%

form

powder

technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable
cell culture | plant: suitable

mp

176 °C (dec.) (lit.)

solubility

H2O: 294 mg/mL, clear, colorless

SMILES string

O.O.Cl[Ca]Cl

InChI

1S/Ca.2ClH.2H2O/h;2*1H;2*1H2/q+2;;;;/p-2

InChI key

LLSDKQJKOVVTOJ-UHFFFAOYSA-L

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General description

Calcium chloride (CaCl2) is a commonly used reagent in biochemistry. It plays important roles in many biological processes, including signal transduction, muscle contraction, and maintenance of cell membrane and cell wall stability. CaCl2 is used in the preparation and transformation of competent Escherichia coli (E. coli) and the transfection of eukaryotic cells with either plasmid DNA or high molecular weight genomic DNA. The CaCl2-mediated electroporation of E. coli with the plasmid DNA pBR322 has been studied. CaCl2 is used in the stabilization and two-dimensional crystallization of the NADH-ubiquinone oxidoreductase (complex I) from E. coli. The crystallization of porcine pancreatic elastase in the presence of CaCl2 along with sodium citrate reveals the binding of calcium in the metal-binding site of the protein. The effect of CaCl2 on the total fluorescence in the polymerization of the tubulin-like filamenting temperature-sensitive mutant Z (FtsZ) division protein of E. coli has been studied by a multiwell fluorescent assay.

Application

Calcium chloride dihydrate has been used:
  • in the formulation of media
  • in sperm collection and preparation
  • in cell culture and protoplast preparation

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificados de análisis (COA)

Lot/Batch Number
SLCM0421
SLCJ8564
SLCF1048
SLBV4869
SLCD4978

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Hemolymph analysis and evaluation of newly formulated media for culture of shrimp cells (Penaeus stylirostris)
Shimizu C, et al.
In Vitro Cellular & Developmental Biology. Animal, 37(6), 322-329 (2001)
Marco Wallenborn et al.
PloS one, 15(7), e0234986-e0234986 (2020-07-08)
Glioblastoma is a common, malignant brain tumor whose disease incidence increases with age. Glioblastoma stem-like cells (GSCs) are thought to contribute to cancer therapy resistance and to be responsible for tumor initiation, maintenance, and recurrence. This study utilizes both SNP
Biomolecule delivery into canola protoplasts by centrifuging cells with microbubbles
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