C6295
Cell Freezing Medium-DMSO Serum free 1x
sterile-filtered, suitable for cell culture
Sinónimos:
cryopreservation medium
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
Productos recomendados
sterility
sterile-filtered
Quality Level
form
liquid
technique(s)
cell culture | mammalian: suitable
cryopreservation: suitable
impurities
endotoxin, tested
shipped in
dry ice
storage temp.
−20°C
General description
Cell freezing medium is a combination of cryoprotectant DMSO with fetal bovine serum. However serum free medium replaces serum with methylcellulose and poloxamer 188 and other additives like ascorbic acid, α-tocopherol and proline.
Application
Cell Freezing Medium-DMSO Serum free 1x has been used in the freezing of human neural progenitor cells (hNPCs), olfactory ensheathing cells (OECs) and retinal pigment epithelial (RPE) cells.
Other Notes
Complete ready-to-use medium containing Minimum Essential Medium, dimethyl sulfoxide (8.7%) and methyl cellulose. Prepared according to the formulation of Waymouth.
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Acute and delayed transplantation of olfactory ensheathing cells promote partial recovery after complete transection of the spinal cord
Lopez-Vales R, et al.
Neurobiology of Disease, 21(1), 57-68 (2006)
Simple freezing procedure for storage in serum-free media of cultured and tumor cells of mouse.
Waymouth, C. and Varnum, D.
TCA Manual, 2(1), 311-313 (1976)
Cryopreservation of cells using defined serum-free cryoprotective agents
Volbers JC, et al.
Current Directions in Biomedical Engineering, 2(1), 315-318 (2016)
A novel serum-free method for culturing human prenatal retinal pigment epithelial cells
Gamm DM, et al.
Investigative Ophthalmology & Visual Science, 49(2), 788-799 (2008)
Glial cell line-derived neurotrophic factor-secreting human neural progenitors show long-term survival, maturation into astrocytes, and no tumor formation following transplantation into the spinal cord of immunocompromised rats.
Gowing G, et al.
Neuroreport, 25(6), 367-367 (2014)
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