C2888
Acetilcolinesterasa from Electrophorus electricus (electric eel)
Type V-S, lyophilized powder, ≥1,000 units/mg protein
Sinónimos:
AChE, Acetil colinesterasa, Acetilcolina acetilhidrolasa
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About This Item
Productos recomendados
biological source
Electrophorus electricus
type
Type V-S
form
lyophilized powder
specific activity
≥1,000 units/mg protein
mol wt
280 kDa
composition
Protein, ≥45% biuret
pI
~5.3
solubility
Tris buffer: 1 mg/mL (0.02 M Tris buffer, pH 7.5)
storage temp.
−20°C
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General description
Peso molecular: 280 kDa
Punto isoeléctrico: 5,5
Coeficiente de extinción: E1% = 18,0 (280 nm)
La acetilcolinesterasa de Electrophorus electricus es un tetrámero compuesto de 4 subunidades iguales de 70 kDa cada una. Cada subunidad contiene un sitio activo. La enzima es una glucoproteína que contiene hexosaminas.
Punto isoeléctrico: 5,5
Coeficiente de extinción: E1% = 18,0 (280 nm)
La acetilcolinesterasa de Electrophorus electricus es un tetrámero compuesto de 4 subunidades iguales de 70 kDa cada una. Cada subunidad contiene un sitio activo. La enzima es una glucoproteína que contiene hexosaminas.
Application
Acetylcholinesterase from Electrophorus electricus (electric eel) has been used:
- in acetylcholinesterase activity assay
- to study its effects on Xenopus embryonic hippocampal cells
- to study the effect of acetylcholine inhibitors on development and plasticity
Biochem/physiol Actions
Acetylcholinesterase (AChE) exhibits its activity against acetylcholine in the neuromuscular junctions and synaptic cleft of cholinergic neurons. It participates in the nitric oxide signaling pathway.
La principal enzima degradadora de la acetilcolina in vivo. Convierte la acetilcolina + H2o en colina + ácido acético.
Caution
For dilute solutions (<1 mg/ml), add 1 mg/ml BSA to stabilize. Solutions containing at least 1 mg protein/ml in dilute neutral phosphate buffer are stable at least 6 months refrigerated.
Unit Definition
One unit will hydrolyze 1.0 μmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C.
Physical form
Lyophilized powder containing Tris buffer salts
Analysis Note
The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Daniela Pereira et al.
Marine drugs, 19(1) (2020-12-31)
Over the last decades, antifouling coatings containing biocidal compounds as active ingredients were used to prevent biofouling, and eco-friendly alternatives are needed. Previous research from our group showed that polymethoxylated chalcones and glycosylated flavones obtained by synthesis displayed antifouling activity
Silvia Olivera et al.
Molecular and cellular neurosciences, 23(1), 96-106 (2003-06-12)
Here we show that chronic application of low concentrations (0.01-0.05 U/ml) or a single application of 1-5 U/ml acetylcholinesterase (AChE) promotes the extension of neuronal processes, synapse formation, and alpha-amino-3-hydroxy-5-methylisoxazolepropionate receptor (AMPAR) surface expression in both embryonic and postnatal hippocampal
Daniel A S Kitagawa et al.
Journal of enzyme inhibition and medicinal chemistry, 36(1), 1370-1377 (2021-06-22)
Organophosphorus poisoning caused by some pesticides and nerve agents is a life-threating condition that must be swiftly addressed to avoid casualties. Despite the availability of medical countermeasures, the clinically available compounds lack a broad spectrum, are not effective towards all
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ChemMedChem, 12(13), 1055-1069 (2017-06-13)
Four mononuclear [(L-L)2 Ru(tatpp)]2+ and two dinuclear [(L-L)2 Ru(tatpp)Ru(L-L)2 ]4+ ruthenium(II) polypyridyl complexes (RPCs) containing the 9,11,20,22-tetraazatetrapyrido[3,2-a:2',3'-c:3'',2''-l:2''',3'''-n]pentacene (tatpp) ligand were synthesized, in which L-L is a chelating diamine ligand such as 2,2'-bipyridine (bpy), 1,10-phenanthroline (phen), 3,4,7,8-tetramethyl-1,10-phenanthroline (Me4 phen) or 4,7-diphenyl-1,10-phenanthroline
Neda Rafat et al.
Biosensors, 11(11) (2021-11-26)
A novel, integrated experimental and modeling framework was applied to an inhibition-based bi-enzyme (IBE) electrochemical biosensor to detect acetylcholinesterase (AChE) inhibitors that may trigger neurological diseases. The biosensor was fabricated by co-immobilizing AChE and tyrosinase (Tyr) on the gold working
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