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C0794

Sigma-Aldrich

Chitosanase from Streptomyces sp.

buffered aqueous glycerol solution, ≥15 units/mg protein (E1%)

Sinónimos:

Chitosan N-acetylglucosaminohydrolase

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About This Item

Número de CAS:
51570-20-8
Comisión internacional de enzimas:
3.2.1.132 (BRENDA, IUBMB)
MDL number:
MFCD00211797
UNSPSC Code:
12352204
NACRES:
NA.54

form

buffered aqueous glycerol solution

Quality Level

200

specific activity

≥15 units/mg protein (E1%)

concentration

≥0.5 mg/mL

solubility

0.1 M acetate buffer, pH 5.5: soluble

storage temp.

−20°C

Categorías relacionadas

Application

Chitosanase from Sigma has been used for polarized light-stimulated, enzymatic hydrolysis of chitin and chitosan.

Biochem/physiol Actions

Chitosanase catalyzes the endohydrolysis of β-1,4-linkages between D-glucosamine (GlcN-GlcN) residues in chitosan. The enzyme from Streptomyces has been reported to also hydrolyze the GlcNAc-GlcN linkage in partially acetylated chitosan.

Unit Definition

One unit will liberate 1.0 μmole of reducing sugar (measured as D-glucosamine equivalents) from chitosan per minute at pH 5.5 at 37 °C.

Physical form

Solution in 50% glycerol containing 50 mM sodium acetate

Preparation Note

Soluble in 10mL of 0.1 M Acetate buffer at pH 5.5

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Sigma-Aldrich

50494

Chitosan from shrimp shells

Quitosano from shrimp shells, ≥75% (deacetylated)

Sigma-Aldrich

C3646

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Sigma-Aldrich

448869

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Quitosano from shrimp shells, practical grade

Sigma-Aldrich

417963

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Sigma-Aldrich

448877

Quitosano

Anna Konieczna-Molenda et al.
Carbohydrate research, 343(18), 3117-3119 (2008-10-01)
Illumination with white linearly polarized light (WLPL) stimulated chitinase and chitosanase in their degradation of chitin and chitosan, respectively. Enzymes were illuminated at room temperature in separate vessels, then admixed in reactors containing polysaccharides. Hydrolysis of chitosan to glucosamine followed
A new reaction for colorimetric determination of carbohydrates.
M Lever
Analytical biochemistry, 47(1), 273-279 (1972-05-01)
T Fukamizo et al.
The Biochemical journal, 311 ( Pt 2), 377-383 (1995-10-15)
Chitosanase was produced by the strain of Streptomyces lividans TK24 bearing the csn gene from Streptomyces sp. N174, and purified by S-Sepharose and Bio-Gel A column chromatography. Partially (25-35%) N-acetylated chitosan was digested by the purified chitosanase, and structures of
Boucher, I., et al.
The Journal of Biological Chemistry, 270, 31-31 (1995)
Ryszard Brzezinski
Bioengineered bugs, 2(4), 226-229 (2011-08-11)
There is a growing interest in chitosanases as enzymatic tools to hydrolyze chitosan into bioactive forms: low molecular weight chitosan (LMWC) or chitosan oligosaccharides (CHOS). However chitosanases are still expensive and methods of large-scale production of these enzymes are not
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Artículos

Enzymatic Assay of Chitosanase from Streptomyces Species

Enzymatic Assay of Chitosanase from Streptomyces Species

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