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Merck

A8400

Sigma-Aldrich

Anti-Human Polyvalent Immunoglobulins (G,A,M)−Peroxidase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:5,000-1:15,000 using IgG, IgA, IgM

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Human immunoglobulins are glycoproteins that regulate immune responses to infections and allergies. Anti-human polyvalent immunoglobulins can be used for serological analyses in diseased population groups . These antibodies may also be used for studying xenotransplantation models and immunological disorders such as rheumatoid arthritis . Anti-Human Polyvalent Immunoglobulins (G,A,M)-Peroxidase antibody is specific for human IgG, IgA and IgM when tested against human IgA, IgG, IgM, and Bence Jones κ and λ myeloma proteins.

Immunogen

Purified human IgA, IgG, and IgM

Application

Anti-Human Polyvalent Immunoglobulins (G,A,M)-Peroxidase antibody is suitable for use in ELISA and immunoblot.
ELISA was performed to determine serotype-specific antibody avidity indexes for sera and monoclonal antibodies using HRP-conjugated goat anti-human polyvalent IgG with incubation for 2 hours.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin with preservative.

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Warning

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves


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We have been studying the native humoral immune response to cancer and have isolated a library of fully human autoantibodies to a variety of malignancies. We previously described the isolation and characterization of two fully human monoclonal antibodies, 27.F7 and

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