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Merck
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Key Documents

81349

Sigma-Aldrich

Poly(2′-deoxyinosinic-2′-deoxycytidylic acid) sodium salt

free from low molecular weight nucleotides, lyophilized

Sinónimos:

Poly(dI-dC) sodium salt

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About This Item

Número de CAS:
118578-37-3
MDL number:
MFCD00147947
UNSPSC Code:
12352106
NACRES:
NA.52

grade

for molecular biology

Quality Level

100

form

powder

quality

lyophilized

mol wt

Mr ~2.5 × 106 (broad molecular weight distribution)
Mr ~2500000

packaging

ampule of ≥10 units A260

storage temp.

−20°C

General description

Poly (2′-deoxyinosinic-2′-deoxycytidylic acid) sodium salt is an alternating copolymer that acts as a substrate for DNA methyltransferases.

Application

Poly(2′-deoxyinosinic-2′-deoxycytidylic acid) sodium salt has been used in electrophoretic mobility shift assay (EMSA) of the transcription factor AP-2 Alpha (TFAP2A) and in southwestern blotting of human embryonic kidney cells (HEK293) nuclear extract.
Suitable for
  • non-specific blocking agent in EMSA
  • evaluation of DNA methyltransferases
  • DNA-small molecule or drug interaction studies

Biochem/physiol Actions

Poly(2′-deoxyinosinic-2′-deoxycytidylic acid), poly(dI-dC) is a synthetic DNA substrate. At high salt, Poly(dI-dC) exists in left handed helical conformation and reverts to right-handed form upon decreasing salt. It is used as a non-specific competitor in electrophoretic mobility shift assay (EMSA).

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Repeated probing of Southwestern blots using alkaline phosphatase stripping
Jia Y, et al.
Journal of Chromatography A, 1217(45), 7177-7181 (2010)
TFAP2A is a component of the ZEB1/2 network that regulates TGFB1-induced epithelial to mesenchymal transition
Dimitrova Y, et al.
Biology Direct, 12(1), 8-8 (2017)
An enzymatic method for the determination of dCTP and dGTP in picomole amounts.
L Skoog
European journal of biochemistry, 17(2), 202-208 (1970-12-01)
Optimization of Competitor Poly (dI-dC) Poly (dI-dC) Levels is Advised in DNA-Protein Interaction Studies Involving Enriched Nuclear Proteins
Larouche K, et al.
Biotechniques, 20(3), 439-444 (1996)
Xiaomei Xu et al.
eLife, 9 (2020-08-09)
Local activation and long-range inhibition are mechanisms conserved in self-organizing systems leading to biological patterns. A number of them involve the production by the developing cell of an inhibitory morphogen, but how this cell becomes immune to self-inhibition is rather
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