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Merck
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Documentos clave

63301-011

Sigma-Aldrich

PCRboost®

1 mL bottle, for improved PCR performance

Sinónimos:

PCRboost®

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.52

form

liquid

usage

28 reactions

storage temp.

room temp

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General description

PCRboost® is a unique reagent that enhances end-point and reverse transcription-PCR performance by improving sensitivity and specificity during amplification of genomic DNA or RNA templates. To use, simply replace the water in your PCR with PCRboost®. That′s all. It is designed to enhance the amplification of DNA without the need for time consuming optimizations. PCRboost® successfully amplifies thermally degraded DNA resulting in a visible amplicon that can be used for downstream applications such as cloning and sequencing.

Don′t see what you are looking for? Custom stabilization services for molecular diagnostic assay and reagent manufacturers are available through Sigma′s partner, Biomatrica. PCRstable® enables the preservation of PCR-based assay components in any format (liquid or dry).

Features and Benefits

Boost PCR results for:

  • Degraded FFPE samples and bisulphite-treated samples (methylation studies)
  • Multiplex PCR
  • Low-copy transcripts
  • Samples containing inhibitory factors (i.e. humic acid, heme, and indigo dye)

No changes to your PCR protocol. Simply replace the water in 
your reaction.

PCR products are compatible with all downstream applications.

Packaging

1 bottle

Physical form

1.0 mL PCRboost® in a 1.5 mL screw-top conical tube

Legal Information

PCRboost is a registered trademark of Biomatrica
PCRstable is a registered trademark of Biomatrica

Related product

Referencia del producto
Descripción
Precios

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Protocolos

Successful Amplification of Thermally Degraded DNA Using PCRboost™

DNA samples are often exposed to harsh environmental conditions, including heat, humidity, UV or chemicals. Some procedures, such as the extraction of samples from formalin-fixed paraffin embedded tissue samples, subject DNA to multiple extreme conditions.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

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