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Merck
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Key Documents

335M-9

Sigma-Aldrich

Cytokeratin (35betaH11) Mouse Monoclonal Antibody

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

100
500

conjugate

unconjugated

antibody form

culture supernatant

antibody product type

primary antibodies

clone

35betaH11, monoclonal

description

For In Vitro Diagnostic Use in Select Regions (See Chart)

form

buffered aqueous solution

species reactivity

human

packaging

vial of 0.1 mL concentrate (335M-94)
vial of 0.5 mL concentrate (335M-95)
bottle of 1.0 mL predilute (335M-97)
vial of 1.0 mL concentrate (335M-96)
bottle of 7.0 mL predilute (335M-98)

manufacturer/tradename

Cell Marque™

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:500

isotype

IgMκ

control

prostate

shipped in

wet ice

storage temp.

2-8°C

visualization

cytoplasmic

Gene Information

human ... KRT1(3848)

General description

Anti-Cytokeratin 8 stains most non-squamous epithelial tumors, squamous tumors are negative for this antibody as a rule. This antibody stains adenocarcinomas of the breast, ovary, gastrointestinal tract, thyroid, pancreas, bile duct, and salivary glands. This antibody does not react with skeletal muscle or nerve cells.

Quality


IVD
IVD
IVD
RUO

Linkage

Cytokeratin (35betaH11) Positive Control Slides, Product No. 335S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Preparation Note

Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.

Other Notes

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Legal Information

Cell Marque is a trademark of Merck KGaA, Darmstadt, Germany

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A C Knapp et al.
Cell, 59(1), 67-79 (1989-10-06)
Intermediate filaments (IFs) of the cytokeratin (CK) type are cytoskeletal elements typical for epithelial differentiation. However, in diverse transformed culture lines of non-epithelial origin, rare cells emerge spontaneously, which synthesize, in addition to their vimentin IFs, CKs 8 and 18.
R Eichner et al.
The Journal of cell biology, 98(4), 1388-1396 (1984-04-01)
Human epidermal keratinocytes express under various growth conditions a total of at least nine keratins that can be divided into two subfamilies. Subfamily A comprises 40-, 46-, 48-, 50-/50'-, and 56.5-kilodalton (kd) keratins which are relatively acidic (pI less than
A M Gown et al.
American journal of clinical pathology, 84(4), 413-424 (1985-10-01)
A panel of monoclonal antibodies to human intermediate filament proteins was tested on an unselected series of 246 neoplasms. The antibody panel includes two different anti-cytokeratin antibodies, an anti-vimentin antibody, and an anti-neurofilament antibody (Gown and Vogel, Am J Pathol
T T Sun et al.
The Journal of investigative dermatology, 81(1 Suppl), 109s-115s (1983-07-01)
Keratins are a group of water-insoluble proteins (molecular weight range 40-70 K) that form 10-nm tonofilaments in a wide variety of epithelial cells. The subunit composition of the keratin filaments varies with cell type, period of embryonic development, stage of
H Battifora
The American journal of surgical pathology, 12 Suppl 1, 24-42 (1988-01-01)
The past few years have seen great progress in our knowledge of the biology and the chemistry of intermediate filaments and other filamentous proteins. This has translated into the development of antibodies that, together with new, highly sensitive immunohistochemical methods

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