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Documentos clave

12080201

L5178Y tk+/- 3.7.2c (IVGT)

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., from mouse thymus

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About This Item

UNSPSC Code:
41106514
NACRES:
NA.81

biological source

mouse thymus

description

Mouse DBA/2 lymphoma L5178Y tk+/-

packaging

tube of 5 μg 12080201-DNA-5UG
pkg of vial of cells 12080201-1VL

growth mode

Suspension

morphology

Small rounded cells

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Cell Line Description

The L5178Y tk+/- 3.7.2c (IVGT) mouse lymphoma cell line was derived from a thymic tumour induced in a DBA/2 mouse by methylcholanthrene treatment. The cell line is tumourigenic in DBA/2 mice. The cell line can be used in genotoxicity tests including gene mutation assays at the tk and other loci and micronucleus induction.

Packaging

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

Culture Medium

Fischer′s medium + 2mM Glutamine + 1% Sodium pyruvate + 10% heat-inactivated Horse Serum (HS)

Subculture Routine

Maintain cultures in logarithmic growth phase between 3 x 104 and 1 x 106 cells/ml.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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