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Merck

SE100

Sigma-Aldrich

Silver Enhancer Kit

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About This Item

UNSPSC Code:
12171500
NACRES:
NA.47

form

liquid

Quality Level

usage

sufficient for ≥100 slides staining and fixing

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

Application

Silver Enhancer Kit has been used to determine the presence of gold nanorods in various tissues and to prepare silver enhanced gold colloids for electron microscopy.

Features and Benefits

Colloidal gold labels are normally visible only at the electron microscope level. Silver Enhancer enlarges the gold colloid label by precipitation of metallic silver to give a high contrast signal visible under a light microscope. A darkroom is not necessary for this procedure, as the silver enhancer working solution is light-stable for 20-30 minutes under ordinary laboratory lighting.

Packaging

The Silver Enhancer kit consists of Solution A (silver salt), Solution B (initiator), Sodium Thiosulfate (fixer), and a Technical Bulletin describing their use.

Principle

Solution A and Solution B are mixed 1:1 immediately before use and applied to the slide holding the gold-labeled section. After 5-10 minutes, the slide is rinsed with distilled water, fixed for 2-3 minutes in a sodium thiosulfate solution, and rinsed again. The section is now ready for counterstaining and mounting as required.

Solo componentes del kit

Referencia del producto
Descripción

  • Sodium thiosulfate pentahydrate, reagent grade, ≥99.5% 500 g

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS

  • S5020Silver enhancer solution A 100 mLSDS

  • S5145Silver enhancer solution B 100 mLSDS

pictograms

Health hazardEnvironment

signalword

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Repr. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

flash_point_f

Not applicable

flash_point_c

Not applicable


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Using Immuno-Scanning Electron Microscopy
for the Observation of Focal Adhesion-substratum
interactions at the Nano- and Microscale in S-Phase Cells
Manus J.P. Biggs
Cell Culture Labfax (2011)
Kathleen Grennan et al.
Talanta, 68(5), 1591-1600 (2008-10-31)
With lower limits of detection and increased stability constantly being demanded of biosensor devices, characterisation of the constituent layers that make up the sensor has become unavoidable, since this is inextricably linked with its performance. This work describe the optimisation
Tsung-Ting Tsai et al.
Scientific reports, 7(1), 3155-3155 (2017-06-11)
In this study, we investigated the antibacterial activity of silver-coated gold nanoparticles (Au-Ag NPs) immobilized on cellulose paper. Ag NPs are known to have strong antibacterial properties, while Au NPs are biocompatible and relatively simple to prepare. We made the
Kumiko A Percival et al.
Cell reports, 41(2), 111484-111484 (2022-10-13)
Midget and parasol ganglion cells (GCs) represent the major output channels from the primate eye to the brain. On-type midget and parasol GCs exhibit a higher background spike rate and thus can respond more linearly to contrast changes than their
A histological evaluation and in vivo assessment of intratumoral near infrared photothermal nanotherapy-induced tumor regression
Hadiyah N
International journal of nanomedicine (2014)

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