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Merck

C1144

Sigma-Aldrich

Chlorazol Black

Powder

Sinónimos:

4-Amino-3-[[4′-[(2,4-diaminophenyl)azo][1,1′-biphenyl]-4-yl]azo]-5-hydroxy-6-(phenylazo)-2,7-naphthalenedisulfonic acid disodium salt, Chlorazole Black E, Direct Black 38

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About This Item

Fórmula empírica (notación de Hill):
C34H25N9Na2O7S2
Número de CAS:
Peso molecular:
781.73
Colour Index Number:
30235
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

product name

Chlorazol Black,

form

powder

Quality Level

composition

Dye content, ≥45% (based on Nitrogen)

color

dark brown to black

solubility

H2O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].Nc1ccc(\N=N\c2ccc(cc2)-c3ccc(cc3)\N=N\c4c(N)c5c(O)c(\N=N\c6ccccc6)c(cc5cc4S([O-])(=O)=O)S([O-])(=O)=O)c(N)c1

InChI

1S/C34H27N9O7S2.2Na/c35-22-10-15-27(26(36)18-22)41-38-24-11-6-19(7-12-24)20-8-13-25(14-9-20)40-42-32-28(51(45,46)47)16-21-17-29(52(48,49)50)33(34(44)30(21)31(32)37)43-39-23-4-2-1-3-5-23;;/h1-18,44H,35-37H2,(H,45,46,47)(H,48,49,50);;/q;2*+1/p-2/b41-38+,42-40+,43-39+;;

InChI key

XRPLBRIHZGVJIC-UVHCWRHYSA-L

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General description

Chlorazol Black has strong affinity for cellulose and chitin. In plant tissues, it helps to differentiate between cellulose and lignin. It also helps in distinguishing fungal bodies from artefacts due to chitin staining. It is also used as an auxiliary stain for chromosomes.

Application

Chlorazol Black has been used to monitor the infection of mycorrhizal Allium porrum in plant roots. It has also been used for the staining of copepods.

pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Carc. 1B - Eye Irrit. 2 - Repr. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P2 (EN 143) respirator cartridges


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B Beije
Mutation research, 187(4), 227-234 (1987-04-01)
The genotoxic activity of the benzidine-derived azo dye, Direct Black 38 (DB38), was studied in vivo, using two different genetic end-points: unscheduled DNA synthesis in liver (UDS) and bone marrow micronucleus (MN). Exposure times were 12, 24 or 36 h.
Gabriela Carvalho Collazzo et al.
Journal of environmental management, 98, 107-111 (2012-01-20)
The N-doped TiO(2) photocatalyst was prepared by calcination of a hydrolysis product composed of titanium (IV) isopropoxide with ammonia as the precipitator. X-ray diffraction, surface area, XPS and UV-vis spectra analyses showed a nanosized anatase structure and the appearance of
Mustafa Işik et al.
Journal of hazardous materials, 114(1-3), 29-39 (2004-10-30)
An Upflow Anaerobic Sludge Blanket Reactor/Continuous Stirred Tank Reactor was used sequentially to decolorize and mineralize C.I. Direct Black 38 azo dye (3200 mg/L) in a synthetic wastewater containing glucose as co-substrate. At the steady state conditions color was effectively
Cell-wall-bound peroxidase activity in roots of mycorrhizal Allium porrum.
Spanu P and Bonfante-Fasolo P
The New phytologist, 109, 119-124 (1988)
Notes on Microscopical Technique for Zoologists null

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