C0537

5-Carboxyfluorescein

≥99% purity (HPLC), powder

• Sinónimos: 5-FAM
• Fórmula empírica (notación de Hill): C₂₁H₁₂O₇
• Número de CAS: 76823-03-5
• Peso molecular: 376.32
• Beilstein/REAXYS Number: 57037
• MDL number: MFCD00036874
• UNSPSC Code: 12171500
• PubChem Substance ID: 24892258
• NACRES: NA.47

Propiedades

• product name: 5-Carboxyfluorescein, ≥99% (HPLC)
• Quality Level: 200
• assay: ≥99% (HPLC)
• form: powder
• solubility: 0.1 M NaOH: 9.80-10.20 mg/mL, clear to slightly hazy
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-8°C
• SMILES string: OC(=O)c1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35
• InChI: 1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)
• InChI key: NJYVEMPWNAYQQN-UHFFFAOYSA-N

Descripción

Application

5-Carboxyfluorescein has been used as an internal marker in capillary isotachophoresis to quantify the charge-based low density lipoprotein subfractions.

Biochem/physiol Actions

5-Carboxyfluorescein is used for the staining of cells to differentiate between live and dead cells. It helps in monitoring the integrity and intactness of the cell membrane. It is also used for the measurement of intracellular pH. 5-Carboxyfluorescein diacetate is a non-fluorescent molecule which is cleaved by intracellular esterases, thereby giving a green fluorescent product 5-carboxyfluorescein. In viable cells, the fluorescent molecule is impermeable to the cellular membrane.

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)