B9431

Biotin-4-Fluorescein

≥95% purity (HPLC), powder

• Sinónimos: 5-(6)-[2-(biotinoyl)aminoethylaminocarbonyl] fluorescein, B4F, Biotin (5-fluorescein) conjugate
• Fórmula empírica (notación de Hill): C₃₃H₃₂N₄O₈S
• Peso molecular: 644.69
• MDL number: MFCD09264223
• UNSPSC Code: 12171500
• eCl@ss: 34058011
• PubChem Substance ID: 329773319
• NACRES: NA.47

Propiedades

• Nombre del producto: Biotin-4-Fluorescein, ≥95.0% (HPLC)
• Quality Level: 100
• assay: ≥95.0% (HPLC)
• form: powder
• mol wt: 644.71
• technique(s): titration: suitable
• color: light orange to very dark orange; light yellow to very dark yellow
• ε (extinction coefficient): ≥70 at 491-497 nm in phosphate buffer at 0.05 M
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: OC(=O)c1cc(ccc1C2=C3C=CC(=O)C=C3Oc4ccc(O)cc24)C(=O)NCCNC(=O)CCCC[C@@H]5SC[C@@H]6NC(=O)N[C@H]56
• InChI: 1S/C33H32N4O8S/c38-18-7-10-25-23(14-18)29(21-9-6-19(39)15-26(21)45-25)20-8-5-17(13-22(20)32(42)43)31(41)35-12-11-34-28(40)4-2-1-3-27-30-24(16-46-27)36-33(44)37-30/h5-10,13-15,24,27,30,38H,1-4,11-12,16H2,(H,34,40)(H,35,41)(H,42,43)(H2,36,37,44)/t24-,27-,30
• InChI key: UROXVNHIYQXTRA-LFERIPGTSA-N

Descripción

Application

Biotin-4-Fluorescein has been used for the labeling of avidin to study the changes in protein binding capability after carbon negative-ion implantation into avidin. It has been used to study the binding affinity of streptavidin towards biotin.

Useful for quantitating biotin binding sites. This reagent binds to avidin more quickly than conventional fluorescein biotin.

Biochem/physiol Actions

Biotin-fluorescein is mainly used for the assay of biotin-interaction sites in avidin and streptavidin. Binding of biotin-4-fluorescein results in strong quenching, which is used for measuring the concentration of (strept)avidin.

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)