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Key Documents

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50484

Sigma-Aldrich

Methylene Blue solution

suitable for microbiology

Sinónimos:

Loeffler’s Methylene Blue

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About This Item

Fórmula empírica (notación de Hill):
C16H18ClN3S
Número de CAS:
61-73-4
Peso molecular:
319.85
Beilstein/REAXYS Number:
3641570
MDL number:
MFCD00012111
UNSPSC Code:
12171500
PubChem Substance ID:
329757603
NACRES:
NA.47

shelf life

limited shelf life, expiry date on the label

concentration

0.5% in H2O (0.5 g/100 mL)

technique(s)

microbe id | staining: suitable

application(s)

microbiology

suitability

Bacillus spp.
Corynebacterium spp.
bacteria

SMILES string

[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

InChI

1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1

InChI key

CXKWCBBOMKCUKX-UHFFFAOYSA-M

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General description

Methylene Blue was earlier recognized as a good nuclear stain, the staining solutions are always either aged or alkalized, if not both. More importantly it is an excellent stain for ribonucleic acid, which is the important stainable constituent of bacterial bodies, of lymphocyte and hemoportoal cytoplasm, and of nerve cell tigroid granules. It can also be employed in capsule stain. The capsule stain begins as a negative stain leaving the organisms colorless. The capsule are clearly seen as halo surrounding the bacterial cell. Another staining procedure is performed with nigrosin (Cat. No. 43925).

Components

reagent contains:
methylene blue 0.5 g
distilled water 100 ml

Replaced by

Referencia del producto
Descripción
Precios

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

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Certificados de análisis (COA)

Lot/Batch Number
BCCG8033
BCCF0773
BCCC6079
BCBZ6621
BCBV2302

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