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Merck

198099

Sigma-Aldrich

Methyl violet 2B

certified by the Biological Stain Commission

Sinónimos:

Basic Violet 1

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About This Item

Número de CAS:
Colour Index Number:
42535
Beilstein/REAXYS Number:
8464808
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

form

crystalline powder

composition

Dye content, 75%

technique(s)

microbe id | staining: suitable

mp

137 °C (dec.) (lit.)

λmax

584 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Cl.C\N=C1/C=C\C(C=C1)=C(/c2ccc(cc2)N(C)C)c3ccc(cc3)N(C)C

InChI

1S/C24H27N3.ClH/c1-25-21-12-6-18(7-13-21)24(19-8-14-22(15-9-19)26(2)3)20-10-16-23(17-11-20)27(4)5;/h6-17H,1-5H3;1H

InChI key

JFTBTTPUYRGXDG-UHFFFAOYSA-N

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General description

Methyl Violet 2B is a mixture of polymethylated (4,5 and 6) pararosaniline chlorides

Application

Methyl violet 2B has been used to stain human lung fibroblast cells (A549) in in vitro antiviral assay.

Biochem/physiol Actions

Methyl violet 2B is a cationic dye with a triphenylmethane structure. It acts as a selective dye and facilitates the differentiation of Klebsiella pneumoniae from Enterobacter aerogenes and other gram-negative organisms. Methyl violet 2B is harmful if inhaled, swallowed or absorbed through skin, contact may cause damage to skin and eyes. Hence, the dye removal is of great importance.

Suitability

Certified for use in Flemming triple stain with iodine for chromosomes; Newton′s crystal violet-iodine technique for chromatin and nucleoli; Lieb′s amyloid stain.

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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