CHAPS-RO

CHAPS

98% (from N), suitable for dialysis, solubility: >50% (Aqueous solutions)

• Sinónimos: CHAPS, 3-[(3-cholamidopropyl)dimethyl-ammonio]-1-propane sulfonate, 3-[(3-cholamidopropyl)dimethyl-ammonio]-1-propane sulfonate, zwitterionic, Cholamidopropyl-dimethyl-ammonio]-1-propane sulfonate, 3-3-, zwitterionic, cholamidopropyl-dimethyl-ammonio]-1-propane sulfonate, 3-3-, 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate
• Fórmula empírica (notación de Hill): C₃₂H₅₈N₂O₇S
• Número de CAS: 75621-03-3
• Peso molecular: 614.88
• MDL number: MFCD00012116
• UNSPSC Code: 12161900

Propiedades

• description: zwitterionic
• assay: 98% (from N)
• form: crystals
• mol wt: micellar avg mol wt 6150; 614.88 g/mol (anhydrous basis)
• packaging: pkg of 10 g (10810118001); pkg of 50 g (10810126001)
• manufacturer/tradename: Roche
• aggregation number: 10
• technique(s): dialysis: suitable
• CMC: 4 mM; 6 mM (20-25°C)
• transition temp: cloud point >100 °C
• solubility: >50% (Aqueous solutions)
• absorption: <0.06% at 278 nm in water at 1 % (w/v) (Ease of removal*: ++; Note:; * Arbitrary scale ranges from ++ to −)
• storage temp.: 2-8°C
• SMILES string: C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)CC4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C
• InChI: 1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22?,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1
• InChI key: UMCMPZBLKLEWAF-RFCNGIAKSA-N

Descripción

General description

3-[(3-Cholamidopropyl)dimethyl-ammonio]-1-propane sulfonate

Application

CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry.
Useful for solubilizing membrane proteins and breaking protein-protein interactions. CHAPS′ small micellar molecular weight (6,150) and high critical micelle concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilization for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 2-4% (w/v) are typically used in an IEF gel.

Solubilization of membrane proteins. Combines the properties of both the sulfobetaine-type and bile-acid detergents.

Physical form

Crystals

Preparation Note

Working concentration: PAGE: 6.5 to 13 mM
Storage conditions (working solution): Do not store in solution, because precipitation may occur.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Información de seguridad

• pictograms: GHS07
• signalword: Warning
• hcodes: H302 - H315 - H319
• pcodes: P264 - P270 - P280 - P301 + P312 + P330 - P337 + P313 - P501
• Hazard Classifications: Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable