11179179001
Roche
Collagen
from rat tail tendon
Sinónimos:
collagen
About This Item
Productos recomendados
biological source
rat tail
Quality Level
sterility
sterile
form
lyophilized (clear, colorless solution after reconstitution)
packaging
pkg of 30 mg
manufacturer/tradename
Roche
technique(s)
cell culture | mammalian: suitable
UniProt accession no.
storage temp.
2-8°C
Gene Information
rat ... Col1a1(29393)
General description
Specificity
Application
Biochem/physiol Actions
Features and Benefits
Lyophilizate, cell culture grade, 3 vials of 10mg
Specifications
Preparation Note
5 μg/cm2 for the coating of cell culture vessels; for the preparation of collagen gels, a final concentration of 2 to 3 mg/ml is used.
Reconstitution
For the preparation of collagen gels, the content of the bottle should be dissolved in 3.3 ml sterile 0.2% acetic acid (v/v) each. This results in a final concentration of 3 mg/ml.
For coating culture dishes the final concentration should be 1 to 2 mg/ml.
Note: For dissolving: do not stir, just pour the acetic acid onto the lyophilizate and allow it to stand for several hours until it has dissolved. To completely dissolve the product an incubation for up to a maximum of 24 hours at 15 to 25 °C may be required.
Reconstitution of the Collagen should be done at 15 to 25 °C, higher temperatures destroy the fibres and may prevent the subsequent gelation of the Collagen.
Other Notes
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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