QIA87

Cytochrome c Release Apoptosis Assay Kit

• UNSPSC Code: 41116133

Propiedades

• usage: sufficient for 100 tests
• Quality Level: 100
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• technique(s): immunoblotting: suitable
• input: sample type cell extract(s)
• storage temp.: −20°C

Descripción

General description

Assay kit provides a unique combination of reagents useful for the isolation of a highly enriched mitochondrial fraction from the cytosol. Translocation of cytochrome c from the mitochondrial fraction to the cytosol is monitored by immunoblotting with the cytochrome c antibody provided with the kit.

Components

Mitochondria Extraction Buffer, Cytosol Extraction Buffer, Protease Inhibitor Cocktail, Reducing Reagent, Cytochrome c Antibody, and a user protocol.

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Specifications

Assay Time: 4 h

Principle

The Cytochrome c Release Apoptosis Assay Kit provides a unique combination of reagents useful for the isolation of a highly enriched mitochondrial fraction from the cytosol that can be used to detect cytochrome c by Western blotting.

Preparation Note

• 1X Cytosol Extraction Buffer: Prepare 1X Cytosol Extraction Buffer by adding the 20 ml 5X Cytosol Extraction Buffer to 80 ml diH₂O; mix well.• Mitochondria Extraction Buffer Mix: Just prior to performing the assay, prepare enough Mitochondria Extraction Buffer Mix for the number of samples to be assayed; each sample requires 0.1 ml of the mix. To prepare 1 ml Mitochondria Extraction Buffer Mix, add 2 µl 500X Protease Inhibitor Cocktail and 1 µl DTT to 1 ml Mitochondria Extraction Buffer; mix well.• Cytosol Extraction Buffer Mix: Just prior to performing the assay, prepare enough Cytosol Buffer Mix for the number of samples to be assayed; each sample requires 1 ml of the mix. To prepare 1 ml Cytosol Extraction Buffer Mix, add 2 µl 500X Protease Inhibitor Cocktail and 1 µl DTT to 1 ml Cytosol Extraction Buffer; mix well.

Storage and Stability

Upon arrival store the entire contents of the kit at -20°C. Once opened, store the Mitochondria Extraction Buffer and Cytosol Extraction Buffer at 4°C and the remaining components at -20°C.

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Storage Class: 8A - Combustible corrosive hazardous materials