QIA113

Caspase-8 Detection Kit (FITC-IETD-FMK)

• UNSPSC Code: 41116133
• NACRES: NA.84

Propiedades

• usage: sufficient for 100 tests
• Quality Level: 100
• species reactivity (predicted by homology): mammals
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles; protect from light
• technique(s): flow cytometry: suitable
• input: sample type intact cells
• fluorescence: λ_ex ~485 nm; λ_em ~535 nm
• detection method: fluorometric
• storage temp.: −20°C

Descripción

General description

A fast, convenient, and sensitive method to measure caspase-8 in living cells. The fluorescent marker, FITC-IETD-FMK, is a labeled, cell-permeable, non-toxic inhibitor that binds irreversibly to activated caspase-8 in living cells. Caspase-8 can be detected by the measurement of fluorescence in a fluorescent microplate reader, flow cytometer, or by fluorescence microscopy.

Specificity

A broad range of species

Components

Labeled Caspase-8 Inhibitor (FITC-IETD-FMK), Wash Buffer, Unlabeled Caspase Inhibitor (Z-VAD-FMK), and a user protocol.

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Specifications

Assay Time: 1.5 h

Storage and Stability

Upon arrival store the entire contents of the kit at -20°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Storage Class: 10 - Combustible liquids