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PICM0RG50

Millipore

Plaquitas verticales para cultivo celular Millicell®

pore size 0.4 μm, diam. 30 mm, transparent PTFE membrane, hydrophilic, H 5 mm, size 6 wells, sterile

Sinónimos:

Millicell Cell Culture Insert, 30 mm, hydrophilic PTFE, 0.4 µm, Millicell-CM, cell culture inserts, organotypic inserts, plate inserts

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About This Item

UNSPSC Code:
41104923
eCl@ss:
32011202
NACRES:
NB.22

material

polystyrene housing
transparent PTFE membrane

Quality Level

400

sterility

ethylene oxide treated
sterile

feature

hydrophilic

manufacturer/tradename

Millicell®

packaging

pkg of Individually blister packaged

parameter

50 °C max. temp.

technique(s)

cell attachment: suitable
cell culture | mammalian: suitable
cell differentiation: suitable

H

5 mm

diam.

30 mm

size

6 wells

surface area

4.2 cm2

color

transparent membrane, when wetted

matrix

Biopore™

pore size

0.4 μm

binding type

low binding surface

detection method

fluorometric

shipped in

ambient

General description

Con las plaquitas Millicell, las células fijadas o en suspensión pueden acceder a los medios desde sus lados apical y basolateral. Crecimiento, estructura y función celulares que imitan más estrechamente lo que ocurre in vivo. Además, las plaquitas Millicell hacen posible el estudio de ambos lados de la monocapa celular.

Application

  • Fijación celular, cultivo celular en 3D, crecimiento celular, diferenciación celular, inmunocitoquímica
  • La membrana Biopore (PTFE hidrófilo) es ideal para baja adsorción proteica, visualización de células en vivo y aplicaciones inmunofluorescentes.

Features and Benefits

  • Para una elevada viabilidad y un estudio superior de la estructura tridimensional del explante
  • Su menor altura las permite encajar dentro de una placa de Petri convencional
  • La membrana Biopore (PTFE) proporciona una elevada viabilidad—de hasta 40 días—y un excelente transporte de oxígeno transmembrana
  • La membrana es ópticamente transparente y optimizada para el mantenimiento prolongado del explante organotípico

Preparation Note

To ensure proper wetting of the membrane from the basolateral side and tomitigate a chance of bubble formation:
  1. Add assay solution or media to the receiver well: 2 mL for 6 well format orequivalent dimensions.
  2. With forceps, place the insert into the receiver well at a 30°-45° angle togradually wet it out from the basolateral side.
  3. Once edges start to wet out, gently lower the insert until it is fully seatedinto the well.

Legal Information

BIOPORE is a trademark of Merck KGaA, Darmstadt, Germany
Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10-13 - German Storage Class 10 to 13

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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This is a Toluidine Blue Staining protocol listing materials and methods.

Microscopic Examination of Samples

This protocol covers 3 modes for the microscopic examination of cell samples.

Indirect Co-culture of Embryonic Stem Cells with Embryonic Fibroblasts

This page covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.

Basic Indirect Co-culture on Both Sides of Millicell® Cell Culture Insert Membranes

This page covers the basic indirect co-culture procedure on both sides of Millicell cell culture insert membranes.

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