MABN763

Anti-Ubiquilin 2 Antibody, clone 6H9

clone 6H9, from mouse

• Sinónimos: Ubiquilin-2, Chap1, DSK2 homolog, Protein linking IAP with cytoskeleton 2, PLIC-2, hPLIC-2, Ubiquitin-like product Chap1/Dsk2
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 6H9, monoclonal
• species reactivity: human, mouse
• technique(s): immunocytochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_038472
• UniProt accession no.: Q9UHD9
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... UBQLN2(29978)

Descripción

General description

Ubiquilin-2, also known as Chap1, DSK2 homolog, Protein linking IAP with cytoskeleton 2 (PLIC-2 or hPLIC-2), Ubiquitin-like product Chap1/Dsk2, and encoded by the gene UBQLN2/N4BP4/PLIC2, is a protein that appears to modulate the half-life of proteins destined to be degraded by the proteasome. Ubiquilin 2 binds UBE3A and BTRC ubquitin ligases and interacts with the 19S proteasome subunit to mediate its activity. Ubiquilin 2 is localized to the cytoplasm and nucleus, and colocalizes with proteasome. Ubiquilin 2 is expressed in mitotic cells very strongly; expression is low in non-dividing cells. Defects in Ubiquilin 2 may be associated with ALS form 15 a neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis.

Immunogen

Recombinant protein corresponding to human Ubiquilin 2.

Application

Detect Ubiquilin-2 using this mouse monoclonal antibody, Anti-Ubiquilin 2 Antibody, clone 6H9 validated for use in western blotting & ICC.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Ubiquilin 2 in 10 µg of SH-SY5Y and NIH-3T3 cell lysate.
Immunocytochemistry Analysis: A representative lot detected Ubiquilin 2 in HeLa cells.

Quality

Evaluated by Western Blotting in U-251 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Ubiquilin 2 in 10 µg of U-251 cell lysate.

Target description

~66 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing PBS, 50% glycerol, and 0.05% sodium azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2