MABN310

Anti-G-protein coupled receptor 56 (GPR56) Antibody, clone H11

clone H11, from mouse

• Sinónimos: G protein-coupled receptor 56, 7-transmembrane protein with no EGF-like N-terminal domains-1, Protein TM7XN1, G-protein coupled receptor 56
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: H11, monoclonal
• species reactivity: mouse
• species reactivity (predicted by homology): human (based on 100% sequence homology)
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001185823
• UniProt accession no.: Q8K209
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... GPR56(9289)

Descripción

General description

G-protein coupled receptor 56 (GPR56) is a ubiquitous adhesion receptor, which belongs to the G-protein coupled receptor 2 family and LN-TM7 subfamily. It is abundantly expressed in the in thyroid gland, brain, heart, and various tumor cells. Previous studies have suggested that GPR56 may inhibit the progression and metastasis of melanomas by interaction with extracellular-matrix proteins, such as Tissue Transglutaminase. GPR56 also plays a role in regulating VEGF production and angiogenesis via a PKCα-mediated pathway. GPR56 also plays a critical role in the development of the frontal cortex; previous studies have reported that mutations in GPR56 result in disorganized cortical lamination, which is most pronounced in the frontal cortex. This condition is known as bilateral frontoparietal polymicrogyria or BFPP.

Immunogen

N-terminal fragment of mouse GPR56

Application

Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in E14.5 mouse brain tissue. (Luo, R., et al. (2011). Proc Natl Acad Sci U S A. 108(31):12925-12930.)

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in meningeal fibroblasts (MFs). (Luo, R., et al. (2011). Proc Natl Acad Sci U S A. 108(31):12925-12930.)

Research Category
Neuroscience

Research Sub Category
Adhesion (CAMs)

This Anti-G-protein coupled receptor 56 (GPR56) Antibody, clone H11 is validated for use in Western Blotting, ICC, IHC for the detection of G-protein coupled receptor 56 (GPR56).

Quality

Evaluated by Western Blot in brain tissue lysate from E13-E14 mouse.

Western Blot Analysis: 0.5 μg/mL of this antibody detected G-protein coupled receptor 56 in 10 µg of brain tissue lysate from E13-E14 mouse.

Target description

~62 kDa observed. Full-length GPR56 (GPR56FL) migrates at 75 kDa and the cleaved extracellular domain of GPR56 (GPR56ECD) migrates at 62 kDa (J Biol Chem. 2008 May 23;283(21):14469-78).

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Brain tissue lysate from E13-E14 mouse

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable