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Key Documents

MAB463

Sigma-Aldrich

Anti-Estrogen Receptor Antibody, clone B10

ascites fluid, clone B10, Chemicon®

Sinónimos:

ER

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

ascites fluid

antibody product type

primary antibodies

clone

B10, monoclonal

species reactivity

rat, human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG2a

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... HSPG2(3339)

Specificity

The peptide used as the immunogen for this product correspond to the amino-acid sequence 151-165 from human ER alpha receptor. By IP, western, gel retardation and ELISA assays clone B10 do not react with other nuclear receptors.

SPECIES REACTIVITIES:

Does not cross react with chicken ER. Other reactivities have not been evaluated. By sequence homology pig ER is 100% identical, rat is 80% conserved.

Immunogen

Epitope: a.a. 151-165

Application

Immunohistochemistry (frozen sections, microwave oven treated paraffin sections) at 1:1,000-1:2,000

Immunocytochemistry (formaldehyde fixation) at 1:1,000-1:2,000

ELISA at 1:1,000-1:2,000

Western blot analysis at 1:1,000-1:2,000

Immunoprecipitation at 1:1-1:10

Gel super shifts at 1:1-1:10

Optimal working dilutions must be determined by the end user.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Use Anti-Estrogen Receptor Antibody, clone B10 (Mouse Monoclonal Antibody) validated in ELISA, WB, IHC(P) to detect Estrogen Receptor also known as ER.

Physical form

Ascites. Liquid, does not contain any preservative.

Storage and Stability

Maintain at -20°C in undiluted aliquots up to 12 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Evidence for a potential role of estrogen in the penis: detection of estrogen receptor-alpha and -beta messenger ribonucleic acid and protein.
Subrina Jesmin, Chishimba N Mowa, Naoyuki Matsuda, Alaa-Eldin Salah-Eldin, Hiroko Togashi et al.
Endocrinology null
John G Geisler et al.
Diabetes, 51(7), 2158-2169 (2002-06-28)
Type 2 diabetes is characterized by loss of beta-cell mass and concomitant deposition of amyloid derived from islet amyloid polypeptide (IAPP). Previously we have shown that expression of human IAPP (huIAPP) in islets of transgenic mice results in either a
P Bhat-Nakshatri et al.
British journal of cancer, 90(4), 853-859 (2004-02-19)
Oestrogen receptor alpha (ERalpha) is an oestrogen-activated transcription factor, which regulates proliferation and differentiation of mammary epithelial cells by activating or repressing gene expression. ERalpha is a critical prognostic indicator and a therapeutic target for breast cancer. Patients with tumours
The poly(A)-dependent transcriptional pause is mediated by CPSF acting on the body of the polymerase.
Anita Nag,Kazim Narsinh,Harold G Martinson
Nature Structural and Molecular Biology null
Estrogen receptor beta expression in invasive breast cancer.
S Mann, R Laucirica, N Carlson, P S Younes, N Ali, A Younes, Y Li, M Younes
Human Pathology null

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