MAB341

Anti-GABA A Receptor β 2,3 Chain Antibody, clone BD17

clone BD17, Chemicon^®, from mouse

• Sinónimos: GABA(A) receptor subunit beta-2, gamma-aminobutyric acid (GABA) A receptor, beta 2, gamma-aminobutyric acid A receptor, beta 2
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: BD17, monoclonal
• species reactivity: mouse, feline, monkey, bovine
• species reactivity (predicted by homology): rat, human
• manufacturer/tradename: Chemicon^®
• technique(s): immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_000813.2
• UniProt accession no.: P47870
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... GABRB2(2561)

Descripción

General description

Gamma-aminobutyric acid type A (GABAA) receptors are members of the neurotransmitter ligand-gated ion channels that mediate neuronal inhibition on binding GABA. The effects of GABA on GABAA receptors are modulated by a range of therapeutically important drugs, including barbiturates, anaesthetics and benzodiazepines.

Specificity

Reacts with the β-chain of the GABAA receptor.

Immunogen

Epitope: Extracellular domain of the beta2 & beta3 subunits of the GABAA receptor

Purified GABA/benzodiazepine receptor from bovine cortex

Application

Detect GABA A Receptor β 2 using this Anti-GABA A Receptor β 2, 3 Chain Antibody, clone BD17 validated for use in IH, IP & WB.

Immunohistochemistry:
A 10-20 μg/mL concetration of a previous lot was used in IH.

Immunoprecipitation:
A previous lot of this antibody was used in IP.

Western blot:
5-20 µg/mL. Recognizes a 55 kDa band corresponding to the beta chain of the GABAA receptor.

Optimal working dilutions must be determined by end user.

Quality

Evaluated by Western Blot on mouse brain membrane lysates.

Western Blot:
1:500 dilution of this antibody detected GABA A Receptor β 2,3 Chain on 10 μg of mouse brain membrane lysates.

Target description

55 kDa

Linkage

Replaces: AB5952

Physical form

Format: Purified

Purified mouse monoclonal IgG1 liquid in buffer containing 10 mM potassium phosphate buffer pH 7.4 with 150 mM NaCl

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany