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Key Documents

MAB329-C

Sigma-Aldrich

Anti-Synaptophysin Antibody, clone SP15 (Ascites Free)

clone SP15, from mouse

Sinónimos:

Major synaptic vesicle protein p38, Synaptophysin

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

SP15, monoclonal

species reactivity

human, monkey, feline, rat, mouse

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgMκ

NCBI accession no.

UniProt accession no.

target post-translational modification

unmodified

Gene Information

human ... SYP(6855)

General description

Synaptophysin (UniProt P08247; also known as Major synaptic vesicle protein p38) is encoded by the SYP (also known as MRX96, MRXSYP) gene (Gene ID 6855) in human. Synaptophysin is a 38 kDa four-transmembrane glycoprotein on synaptic vesicle (SV) with both its N- and C-termini exposed cytoplasmically. Synaptophysin forms homomultimers in the SV membrane and is heavily phosphorylated on tyrosine residues in its C-terminal pentapeptide repeats. Synaptophysin is also known to interact with SV v-SNARE protein synaptobrevin II (sybII) when sybII is not engaged with other SNARE proteins, suggesting a role of synaptophysin in sybII retrieval during SV endocytosis. Consistently, synaptophysin-knockout neurons display reduced levels of sybII at their nerve terminals.

Immunogen

Human brain proteins immunoprecipitated with EP10 (Cat. No. MAB332).

Application

Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
This Anti-Synaptophysin Antibody, clone SP15 (Ascites Free) is validated for use in Western Blotting, Immunohistochemistry, Immunocytochemistry, ELISA for the detection of Synaptophysin.
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Synaptophysin in 10 µg of human brain tissue lysate.
Western Blotting Analysis: A representative lot detected synaptophysin in rat hippocampal tissue extracts (Lin, D., et al. (2012). Behav. Brain Res. 228(2):319-327).
Western Blotting Analysis: Representative lots detected synaptophysin in mouse brain synaptosomes preparations (Shim, S.Y., et al. (2008). J. Neurosci. 28(14):3604-3614).
Western Blotting Analysis: A representative lot detected synaptophysin in the large dense-core vesicles-containing fractions obtained by sucrose gradient centrifugation of rat brain median eminence (ME) neuroterminals preparations (Yin, W., et al. (2007). Exp. Biol. Med. (Maywood). 232(5):662-673).
Immunohistochemistry Analysis: A representative lot detected a stronger synaptophysin immunoreactivity in the inner molecular layer of frozen dentate gyrus sections from a 3-week old monkey, while a stronger immunoreactivity was seen in the outer molecular layer of dentate gyrus sections from a 13-year old monkey (Lavenex, P., et al. (2007). Dev. Neurosci. 29(1-2):179–192).
Immunohistochemistry Analysis: A representative lot immunostained nerve terminals at palisade ending using cat extraocular muscle (EOM) whole mount sections (Konakci, K.Z., et al. (2005). Invest. Ophthalmol. Vis. Sci. 46(1):155-165).
Immunohistochemistry Analysis: A representative lot detected synaptophysin immunoreactivity in human brain tissue sections (Honer, W.G., et al. (1993). Brain Res. 609(1-2):9-20).
Immunocytochemistry Analysis: A representative lot detected punctate synatophysin immunoreactivity among cultured primary cerebrocortical neurons from rat embryos (Bragina, L., et al. (2006). J. Neurochem. 99(1):134-141.).
ELISA Analysis: Representative lots were employed as the capture antibody for the detection of synaptophysin in human temporal cortex, frontal cortex, and cerebellar cortex extracts by sandwich ELISA (Klucken, J., et al. (2006). Acta Neuropathol. 111(2):101-108; Fukumoto, H., et al. (2002). Arch. Neurol. 59(9):1381-1389).

Quality

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Synaptophysin in 10 µg of rat brain tissue lysate.

Target description

~34 kDa observed

Physical form

Format: Purified
Purified mouse monoclonal IgMκ antibody in PBS with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Optional

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Beta-secretase protein and activity are increased in the neocortex in Alzheimer disease.
Fukumoto, H; Cheung, BS; Hyman, BT; Irizarry, MC
Archives of Neurology null
GLT-1 down-regulation induced by clozapine in rat frontal cortex is associated with synaptophysin up-regulation.
Bragina, L; Melone, M; Fattorini, G; Torres-Ramos, M; Vallejo-Illarramendi, A; Matute, C; Conti, F
Journal of Neurochemistry null
Clinical and biochemical correlates of insoluble alpha-synuclein in dementia with Lewy bodies.
Klucken, J; Ingelsson, M; Shin, Y; Irizarry, MC; Hedley-Whyte, ET; Frosch, M; Growdon et al.
Acta neuropathologica null
Molecular characteristics suggest an effector function of palisade endings in extraocular muscles.
Konakci, KZ; Streicher, J; Hoetzenecker, W; Blumer, MJ; Lukas, JR; Blumer, R
Investigative Ophthalmology & Visual Science null
Postnatal development of the primate hippocampal formation.
Lavenex, P; Banta Lavenex, P; Amaral, DG
Developmental Neuroscience null

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