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Key Documents

MAB312RX

Sigma-Aldrich

Anti-Neuron Cell Surface Antigen Antibody, clone A2B5-105 (7), Alexa Fluor 488

clone A2B5-105 (7), Chemicon®, from mouse

Sinónimos:

A2B5

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

ALEXA FLUOR 488

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

A2B5-105 (7), monoclonal

species reactivity

mouse, chicken, human, rat

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable

isotype

IgM

shipped in

wet ice

target post-translational modification

unmodified

Specificity

The antibody recognizes an epitope common to silaogangliosides and sulfatides from the plasma membranes of neurons, and neuroendocrine and glial cells. Anti-A2B5 has been used to specifically stain:

· Neurofibrillary tangles in the post-mortem brain tissue of patients with a confirmed diagnosis of Alzheimer′s disease (Majocha et al., 1989)

· Neurons of chicken retina, brain, spinal cord, and dorsal root ganglia (Schnitzer & Schachner, 1982; Eisenbarth et al., 1979.

· Rat striatal nerve terminals [synaptosomes (Wolf & Kapatos, 1989].

· Neuroblastoma cells in man (Oppedal et al., 1989; Coakham et al., 1985).

· Rat insulinoma cells and pancreatic islets cells (Shimizu et al., 1983; Bartholomeusz et al., 1989).

· Human and rat thymic epithelial cells (Haynes, et al., 1988).

· Cells involved in gliogenesis (Bottenstein et al., 1988; Suzumura & slerberg, 1989).

· Oligodendrocytes and astrocytes, type II (Dubois-Dalcq et al., 1990).

Immunogen

Embryonic chicken retinal cells

Application

Anti-Neuron Cell Surface Antigen Antibody, clone A2B5-105 (7), Alexa Fluor 488 detects level of Neuron Cell Surface Antigen & has been published & validated for use in IC, IH.
Immunocytochemistry

Immunohistochemistry: 1:30

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers

Physical form

Purified immunoglobulin conjugated to Alexa Fluor 488. Liquid in Phosphate buffer with 15 mg/mL BSA as a stabilizer and 0.1% sodium azide.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots in the dark for up to 6 months after date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Alexa Fluor is a registered trademark of Molecular Probes, Inc.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Visite la Librería de documentos

The effect of TGF-beta2 on elastin, type VI collagen, and components of the proteolytic degradation system in human optic nerve astrocytes.
Neumann, Carolin, et al.
Investigative Ophthalmology & Visual Science, 49, 1464-1472 (2008)
Pancreatic islet A2B5- and 3G5-reactive gangliosides are markers of differentiation in rat insulinoma cells.
Bartholomeusz, R K, et al.
Endocrinology, 124, 2680-2685 (1989)
Flow cytometric analysis of rat striatal nerve terminals
Wolf, M E and Kapatos, G
The Journal of Neuroscience, 9, 94-105 (1989)
B F Haynes et al.
The Journal of clinical investigation, 71(1), 9-14 (1983-01-01)
Using a monoclonal antibody (A2B5), which binds to GQ ganglioside, and tetanus toxin, which binds to GD and GT gangliosides, distinct regions of human and rodent thymic epithelial cells have been identified. The lymphoid elements of the thymus do not
Bone marrow examination in neuroblastoma patients: a morphologic, immunocytochemical, and immunohistochemical study
Oppedal, B R, et al
Human Pathology, 20, 800-805 (1989)

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